Rat monoclonals against Mouse immunoglobulins

Rat IgG2ak - clone LO-MD-6

Rat IgG1k - clone LO-MD-8

Rat IgG2ak - clone LO-MD-6

Specifications

Immunogen :

IgD (Mouse Delta heavy chain of Immunoglobulin) TEPC 1017, TEPC 1033 (1)

Reactivity :

Mouse delta heavy chain of immunoglobulin (determined by immunodot).

Cross-reactivity :

Does not bind to human IgG and IgM. Does not bind to chicken, rabbit, goat, sheep, bovine, horse, dog, swine, baboon IgG (ELISA test).

Avidity (3) :

on Mouse IgD: 1.2 x 10⁹ M^-1 by BIACORE (Pharmacia)*.

Applications :

Cf Reactivity
- capture Elisa: good binding on plastics
- can be labelled with Biotin
- can be labelled with Peroxidase and FITC.
- can be used as second antibody in immunoassays
- can be used in immunoffinity chromatography for purification of mouse IgD (solid phase Sepharose 4b CNBr act.) - capacity: see data sheet.
- can be coated on nitrocellulose (dot-elispot)
- can be used on cryosections (immunohistology) as first antibody labelled with peroxidase

References

Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids 1982, Peeters E. ed., 29th Colloquium 1981, Pergamon Press, Oxford and N.Y., pp. 615- 618.

(1) Finkelman F.O., kessler J.W., Mushinski J.F. and Potter M. IgD-secreting murine plasmacytomas: identification and partial characterization of two IgD myeloma proteins. J. Immunol. 1981, 126: 680.

(3) Van Heymingen et al. J. Immunol. Methods 1983, 62: 147-153.

Selected References of scientific publications in which LO-MD-6 MAb was used: Goroff D.k., Holmes, J.M., Bazin, H., Nisol, F., Finkelman, F.D. J. Immunol., 1991, 146: 18-25 (in this paper, LO-MD-6 is named Hbd-6).; Denis O., Latinne D., Nisol F., Bazin H. Int. Immunol., 1993, 5 (1): 71-78. Latinne D. et al. Immunol. Rev. 1994, 141: 95-125.

*kindly determined by Th. Delaunay (INRA, France).

Rat IgG1k - clone LO-MD-8

Specifications

Immunogen :

IgD (Mouse delta heavy chain of immunoglobulin) TEPC 1017

Reactivity :

Mouse delta heavy chain of immunoglobulin (determined by immunodot)

Avidity (2) :

on mouse IgD: 2.9 x 109 M-1

Applications :

Cf reactivity
- can be labelled with biotin.
- can be used as second antibody in immunoassay
- capture Elisa: good binding on plastics

References

Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids 1982, Peeters E. ed., 29th Colloquium 1981, Pergamon Press, Oxford and N.Y., pp. 615- 618.

(2) Van Heymingen et al. J. Immunol. Methods 1983, 62: 147-153.

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