Rat monoclonals against mouse immunoglobulins

Rat monoclonals against Mouse immunoglobulins

Anti Mouse IgE epsilon heavy chain

Rat IgG2ak - clone LO-ME-2

Format	Cat.	Qty
Purified	UPB90410	0.5ml
Purified	UPB90411	1ml
Biotin	UPB90420	0.5ml
Biotin	UPB90421	1ml
FITC	UPB90430	0.5ml
FITC	UPB90431	1ml
Peroxidase	UPB90440	0.5ml
Peroxidase	UPB90441	1ml

Specifications

Immunogen :	purified mouse IgE from BALB/c mice
Reactivity :	Mouse epsilon heavy chain of immunoglobulin (determined by immunodot) LO-ME-2 and LO-ME-3 do not bind to the same epitope.
Cross-reactivity :	does not bind to human IgG and IgM. Does not bind to chicken, rabbit, goat, sheep, bovine, horse, dog, swine, baboon IgG (ELISA test).
Avidity :	on mouse IgE: 2 x 109 M-1 - Cf. Avidity sheet, for more details
Applications :	Cf reactivity - can be used for capture elisa: good binding on plastics - can be labelled with peroxidase - can be labelled with biotin - can be labelled with FITC - can be used as second antibody in immunoassays - can be used in immunoaffinity chromatography for purification of mouse IgE (solid phase Sepharose 4B CNBr act.).
Lyophilization :	Yes.

References

Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y.: 615-618

Selected Reference : of scientific publication in which LO-ME-2 MAb was used: Chavez M. et al. Int. Arch. All. Immunol. 1992,97:330-336.

Rat IgG1k - clone LO-ME-3

Format	Cat.	Qty
Purified	UPB90450	0.5ml
Purified	UPB90451	1ml
Biotin	UPB90460	0.5ml
Biotin	UPB90461	1ml
FITC	UPB90470	0.5ml
FITC	UPB90471	1ml
Peroxidase	UPB90480	0.5ml
Peroxidase	UPB90481	1ml

Specifications

Immunogen :	purified IgE (Mouse IgE) from BALB/c mice
Reactivity :	Mouse epsilon heavy chain of immunoglobulin (determined by immunodot) LO-ME-2 and LO-ME-3 do not bind to the same epitope. LO-ME-3 doenít cross-react with rat IgE.
Cross-reactivity :	does not bind to human IgG and IgM. Does not bind to chicken, rabbit, goat, sheep, bovine, horse, dog, wine, baboon IgG (ELISA test).
Avidity :	on mouse IgE: 3 x 109 M-1 - Cf. Avidity sheet, for more details
Applications :	Cf reactivity - can be used for capture elisa: good binding on plastics - can be labelled with biotin - can be labelled with peroxidase - can be labelled with FITC - can be used as second antibody in immunoassays - can be coated on nitrocellulose (dot-elispot)
Lyophilization :	yes

References

Selected References of scientific publications in which LO-ME-3 MAb was used Chavez M. et al. Int. Arch. All. Immunol. 1992,97:330-336, El Bouhdidi et al. Parasite Immunology 1994, 16: 69-76; Pierre P. et al. Eur. J. Immunol., 1992, 22: 3179-3182; Van Mechelen M. et al. Int. Immunol. 1995, 7:199-205.

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