Rat monoclonals against Mouse immunoglobulins

Rat IgG1k - clone LO-MG1-2

Rat IgG1k - clone LO-MG1-2

Reactivity :

Mouse gamma1 heavy chain of immunoglobulin (determined by immunodot), positively tested on BALB/c and C5781/7 mouse sera.
The specificity of every rat monoclonal antibody anti-mouse IgG subclasses is determined in a range of optimal concentrations. Increasing the first or the second antibody at an unnecessary too high concentration can induce possible cross-reactions with other mouse IgG subclasses.
LO-MG1-2 and LO-MG1-13 do not bind to the same epitope.

Avidity :

on mouse IgG1: 9 x 10⁸ M^-1 - Cf. Avidity sheet, for more details

Cross-reactivity :

does not cross react with a normal serum of rat and rat IgG1, IgG2a, IgG2b, IgM and IgA secreting hybridomas supernatants. It cross-reacts with highly concentrated rat IgG2c supernatant. It does not bind to chicken, rabbit, goat, sheep, bovine, horse, dog IgG and human IgM. It binds to swine, human IgG and weakly baboon IgG (ELISA test).

Applications :

Cf Reactivity
- can be labelled with FITC
- can be labelled with biotin
- can be labelled with peroxidase
- can be used as second antibody in immunoassays
- can be used for capture elisa: good binding on plastics
- can be used in mmunoaffinity chromatography for purification of mouse IgG1 (Solid phase Sepharose 4b CNBr act.) - capacity: see data sheet.

Lyophilization :

Yes, with a low possible denaturation.

References

Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed.,29th collloquium 1981 Pergamon Press Oxford and N.Y.: 615-618

Selected References of scientific publications in which LO-MG1-2 MAb was used : Pierre P. et al. Eur. J. Immunol., 1992, 22: 3179-3182; Denis O. et al. Int. Immunol., 1993, 5 (1): 71-78; El Bouhdidi et al. Parasite Immunology 1994, 16: 69-76; Vanhove B., Bazin H. Immunology 1993, 79: 580-586.

Rat IgG1k - clone LO-MG1-13

Immunogen :

purified mouse IgG1 from BALB/c mice

Reactivity :

Mouse gamma1 heavy chain of immunoglobulin (determined by immunodot)
The specificity of every rat monoclonal antibody anti-mouse IgG subclasses is determined in a range of optimal concentrations. Increasing the first or the second antibody at an unnecessary too high concentration can induce possible cross-reactions with other mouse IgG subclasses.
LO-MG1-2 and LO-MG1-13 do not bind to the same epitope.

Cross-reactivity :

does not bind to human IgG and IgM. Does not bind to chicken, rabbit, goat, sheep, bovine, horse, dog and swine IgG. Weakly cross-reacts with baboon IgG (ELISA test).

Avidity :

on mouse IgG1: 5.1 x 109 M-1 . Cf. Avidity sheet, for more details.

Applications :

Cf Reactivity
- can be used for capture elisa: good binding on plastics
- can be labelled with biotin
- can be labelled with peroxidase and FITC
- can be used as second antibody in immunoassays
- can be coated on nitrocellulose (dot-elispot)

Applications :

- can be used on cryosections (immunohistology) as first (on mouse) or as second antibody (on rat) labelled with peroxidase

Lyophilization :

not tested

References

Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed.,29th collloquium 1981 Pergamon Press Oxford and N.Y.: 615-618

Selected References of scientific publications in which LO-MG1-13 MAb was used: Soares M. et al. Transplantation Proceedings, 1992, 24:51-452; Denis O. et al. Int. Immunol., 1993, 5 (1): 71-78; Soares M. et al. Transplantation 1993, 56: 1427-1433; Van Mechelen M. et al. Int. Immunol. 1995, 7: 199-205.

Rat IgG1k - clone LO-MG1-15

Immunogen :

purified mouse IgG1 from BALB/c mice

Reactivity :

Mouse gamma1 heavy chain of immunoglobulin (determined by immunodot)

The specificity of every rat monoclonal antibody anti-mouse IgG subclasses is determined in a range of optimal concentrations. Increasing the first or the second antibody at an unnecessary too high concentration can induce possible cross-reactions with other mouse IgG subclasses.

Cross-reactivity :

It binds to swine and human IgG. Does not bind to rabbit, bovine IgG or human IgM (ELISA test).

Avidity :

on mouse IgG1: 2.8 x 109 M-1

Applications :

Cf Reactivity
- can be labelled with biotin
- can be labelled with FITC
- can be used as second antibody in immunoassays
- can be used for capture elisa: good binding on plastics

Lyophilization :

not tested

Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed.,29th collloquium 1981 Pergamon Press Oxford and N.Y.: 615-618

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