Rat monoclonals against Mouse immunoglobulins

Rat IgG2ak - clone LO-MG2a-2

Immunogen :

purified IgG from BALB/c mice

Reactivity :

mouse gamma2a heavy chain of immunoglobulin (determined by immunodot)
The specificity of every rat monoclonal antibody anti-mouse IgG subclasses is determined in a range of optimal concentrations. Increasing the first or the second antibody at an unnecessary too high concentration can induce possible cross-reactions with other mouse IgG subclasses.

Cross-reactivity :

does not bind to chicken, rabbit, goat, horse, swine, baboon, and human IgG. Does not bind to human IgM. It binds to dog and weakly sheep and bovine IgG (ELISA test).

Avidity :

on mouse IgG2a: 7 x 109 M-1 - See Avidity sheet, for more details.

Applications :

Cf Reactivity
- can be labelled with FITC
- can be labelled with biotin
- can be labelled with peroxidase
- can be used as second antibody in immunoassays

- can be used for capture elisa: low binding on plastics
- can be used in immunoaffinity chromatography for purification of mouse IgG2a (Solid phase Sepharose 4B CNBr act.)

Lyophilization :

Yes, possibly with a low denaturation.

References

-Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th Colloquium 1981 Pergamon Press, Oxford and N.Y., pp. 615-618

-Selected Reference : of scientific publication in which LO-MG2a-2 MAb was used: El Bouhdidi et al. Parasite Immunology 1994, 16: 69-76

Reactivity :

Mouse gamma2a heavy chain of immunoglobulin (determined by immunodot)
The specificity of every rat monoclonal antibody anti-mouse IgG subclasses is determined in a range of optimal concentrations. Increasing the first or the second antibody at an unnecessary too high concentration can induce possible cross-reactions with other mouse IgG subclasses.

Cross-Reactivity :

does not bind to chicken, rabbit, goat, sheep, bovine, horse, dog, swine and baboon IgG and human IgG or IgM (ELISA test).

Avidity :

on mouse IgG2a: 3 x 109 M-1 - Cf. Avidity sheet, for more details.

Applications :

Cf Reactivity
- can be labelled with biotin and peroxidase
- cannot be easily labelled with FITC
- can be used as second antibody in immunoassays
- can be used for capture elisa: low binding on plastics
- can be used in immunoaffinity chromatography for purification of mouse IgG2a (solid phase Sepharose 4B CNBr act.) - capacity: see data sheet.

Lyophilization :

not tested

References

Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th Colloquium 1981 Pergamon Press, Oxford and N.Y., pp. 615-618.Selected References of scientific publications in which LO-MG2a-3 MAb was used: Pierre P. et al. Eur. J. Immunol., 1992, 22: 3179-3182; Denis O. et al. Int. Immunol., 1993, 5 (1): 71-78.

Immunogen :

purified IgG from BALB/c mice

Reactivity :

Mouse gamma2a heavy chain of immunoglobulin (determined by immunodot)
The specificity of every rat monoclonal antibody anti-mouse IgG subclasses is determined in a range of optimal concentrations. Increasing the first or the second antibody at an unnecessary too high concentration can induce possible cross-reactions with other mouse IgG subclasses.

Cross-reactivity :

does not bind to chicken, rabbit, goat, sheep, bovine, horse, dog, swine, baboon IgG and human IgG or IgM (ELISA test).

Avidity :

On mouse IgG2a: 5.1 x 109 M-1

Applications :

Cf Reactivity
- can be labelled with biotin, FITC and peroxidase
- can be used as second antibody in immunoassays
- can be used for capture elisa: good binding on plastics
- can be coated on nitrocellulose (dot-elispot)

Lyophilization :

not tested

References

-Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th Colloquium 1981 Pergamon Press, Oxford and N.Y., pp. 615-618.

-Selected Reference : of scientific publications in which LO-MG2a-7 Mab was used: Van Mechelin M. et al. Int. Immunol. 1995, 7: 199-205.

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