Rat monoclonals against Mouse immunoglobulins

Rat IgG1l - clone LO-MG2b-1

Rat IgG1k - clone LO-MG2b-2

Immunogen :

polyclonal IgG from BALB/c mice

Reactivity :

Mouse gamma2b heavy chain of immunoglobulin (determined by immunodot)

-

The specificity of every rat monoclonal antibody anti-mouse IgG subclasses is determined in a range of optimal concentrations. Increasing the first or the second antibody at an unnecessary too high concentration can induce possible cross-reactions with other mouse IgG subclasses.

Cross-reactivity :

does not bind to chicken, rabbit, goat, sheep, bovine, horse, dog, swine, baboon IgG and human IgG or IgM (ELISA test).

Avidity :

on mouse IgG2b: 3 x 108 M-1 - Cf. Avidity sheet, for more details.

Applications :

Cf reactivities
- can be used in immunoaffinity chromatography for purification of mouse IgG2b (solid phase Sepharose 4B CNBr act.) - capacity and conditions of elution: see data sheet.
- can be labelled with FITC
- can be used as second antibody in immunoassays
- can be used for capture elisa: middle binding on plastics

Lyophilization :

not tested

Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed. 29th Colloquium 1981, Pergamon Press, Oxford and N.Y., pp. 615-618.

Immunogen :

purified IgG from BALB/c mice

Reactivity :

Mouse gamma2b heavy chain of immunoglobulin (determined by immunodot), positively tested on BALB/c and C57B1/7 mouse sera. The specificity of every rat monoclonal antibody anti-mouse IgG subclasses is determined in a range of optimal concentrations. Increasing the first or the second antibody at an unnecessary too high concentration can induce possible cross-reactions with other mouse IgG subclasses.

Cross-reactivity :

does not bind to chicken, rabbit, goat, sheep, bovine, horse, dog, swine, baboon IgG and human IgG or IgM (ELISA test).

Avidity :

on mouse IgG2b: 1 x 1010 M-1 - Cf. Avidity sheet, for more details

Applications :

Cf Reactivity
- can be used in immunoaffinity chromatography for purification of mouse IgG2b (solid phase Sepharose 4B CNBr act.) - capacity: see data sheet.
- can be labelled with FITC, biotin and peroxidase
- can be used as second antibody in immunoassays
- can be used for capture elisa: low binding on plastics
- can be coated on nitrocellulose (dot-elispot)

Lyophilization :

Yes, possibly with a low denaturation.

References

-Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed. 29th Colloquium 1981, Pergamon Press, Oxford and N.Y., pp. 615-618.

-Selected References of scientific publications in which LO-MG2b-2 MAb was used: Denis O. et al. Int. Immunol., 1993, 5 (1): 71-78; El Bouhdidi et al. Parasite Immunology 1994, 16: 69-76; Pierre P. et al. Eur. J. Immunol., 1992, 22: 3179-3182

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