Rat monoclonals against Mouse immunoglobulins

Rat IgMk - clone MM-3

Rat IgMk - clone LO-MM-8

Rat IgMk - clone MM-3

Immunogen :

purified IgM (Mouse polyclonal IgM) from BALB/c mice

Reactivity :

Mu heavy chain of Mouse immunoglobulin (2) (determined by immunodot).

Avidity :

on mouse IgM: 2 x 108 M-1 - Cf. Avidity sheet, for more details

Cross-reactivity :

Does not bind to rabbit, swine and human IgG. Weak reaction with chicken, goat, sheep, bovine, horse, dog, baboon IgG and with human IgM (ELISA test).

Applications :

Cf Reactivity
- can be used as second antibody in immunoassays
- can be labelled with TRITC
- capture Elisa: good binding on plastics
- can be used in mmunoaffinity chromatography for purification of mouse IgM (Solid phase Sepharose 4B CNBr act.)

Lyophilization :

not tested

Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y.: 615-618

(2) Cormont F., Manouvriez P., De Clercq L., Bazin H. The use of rat monoclonal antibodies to characterize, quantify and purify polyclonal or monoclonal mouse IgM. Meth. in Enzymol. 1986,121:622-631

Rat IgMk - clone LO-MM-8

Immunogen :

purified IgM (Mouse polyclonal IgM) from BALB/c mice

Reactivity :

Mu heavy chain of Mouse immunoglobulin (2) (determined by immunodot).

Avidity :

on mouse IgM: 3.6 x 109 M-1 .Cf. Avidity sheet, for more details

Applications :

Cf Reactivity
- can be used as second antibody in immunoassays
- can be labelled with Peroxidase
- capture Elisa: good binding on plastics
- can be used on cryosections (immunohistology) as first antibody labelled with peroxidase

Lyophilization :

not tested

-Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y.: 615-618;

-(2) Cormont F., Manouvriez P., De Clercq L., Bazin H. The use of rat monoclonal antibodies to characterize, quantify and purify polyclonal or monoclonal mouse IgM. Meth. in enzymol. 1986, 121:622-631

Rat IgG2ak - clone LO-MM-9

Immunogen :

purified IgM (Mouse polyclonal IgM) from BALB/c mice

Reactivity :

Mu heavy chain of Mouse immunoglobulin (2) (determined by immunodot). positively tested on BALB/c and C57B1/7 mouse sera. It binds to an epitope in the cµ3 domain.

Cross-reactivity :

does not bind to human myeloma of IgM, IgG, IgA, IgD, IgE isotypes. Does not bind to chicken, rabbit, goat, sheep, bovine, horse, dog, swine, baboon IgG (ELISA test).

Avidity :

on mouse IgM: 6.8 x 108 M-1 by BIACORE (Pharmacia)*. Cf. Avidity sheet, for more details.

Applications :

Cf Reactivity
- can be labelled with Iodine (2), Fitc and Biotin
- cannot be labelled with Peroxidase, use LO-MM-3
- capture Elisa: good binding on plastics
- can be used as second antibody in immunoassays
- can be used in mmunoaffinity chromatography for purification of mouse IgM (Solid phase Sepharose 4B CNBr act.) - capacity: see data sheet.
- can be coated on nitrocellulose (dot-elispot)
- can be used on cryosections (immunohistology) as first antibody (purified) (3)

Lyophilization :

Yes, but with partial denaturation.

Bazin H. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y.: 615-618;

(2) Cormont F., Manouvriez P., De Clercq L., Bazin H. Meth. in Enzymol. 1986, 121: 622-631; Bazin H., Malache J.M. J. Immunol. Meth. 1986, 88: 19-24;

(3) Latinne et al. Immunol. Rev. 1994, 141:95-125.

Selected References of scientific publications in which LO-MM-9 MAb was used: Bendelac A. et al. J. Immunol., 1988, 141:2625-2628; Cerny A. et al. Transplantation, 1988, 45: 1111-1113; Cerny A. et al. J. Virol. 1988, 62: 1803-1807; Cormont F. et al. Meth. Enzymol. 1986, 121: 622-631; Denis O. et al., Int. Immunol. 1993, 5 (1): 71-78; Latinne D. et al. Immunol. Rev. 1994, 141: 95-125.* kindly determined by Th. Delaunay

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