Rat monoclonals against Mouse immunoglobulins

Rat IgG2ak - clone LO-MK-1

Rat IgG1k - clone LO-MK-2

Rat IgG2ak - clone LO-MK-1

Immunogen :

purified Mouse k IgG1 from BALB/c mice

Reactivity :

Mouse k light chain of immunoglobulin (determined by immunodot).
positively tested on BALB/c and C57B1/7 mouse sera.
LO-Mk-1 and LO-Mk-2 do not bind to the same epitope.

Cross-reactivity :

Does not cross-react with chicken, rabbit, goat, sheep, bovine, horse, dog, swine, baboon, and human IgG and with human IgM (ELISA test).

Avidity :

on mouse IgG2ak: 3 x 109 M-1. Cf. Avidity sheet, for more details

Applications :

Cf Reactivity
- Can be labelled with Peroxidase
- Can be labelled with Fitc
- Can be labelled with Biotin
- Can be used for capture elisa: low binding on plastics
- Second antibody in immunoassays
- Cannot be used for immunoaffinity chromatography for purification of mouse k Ig (no elution at pH 2.8).
- Can be used in immunohistology (as second antibody labelled with peroxidase or FITC)(2).

Lyophilization :

Yes, with a possible low denaturation.

References

Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y.: 615-618

(2) Latinne et al. Immunol. Rev. 1994, 141:95-125.

Selected References of scientific publications in which LO-Mk-1 Mab was used: Delaunay T. et al. J. Immunol. Methods, 1990, 131:33-39.

Denis O. et al. Internat. Immunol., 1993, 5 (1): 71-78.

Rat IgG1k - clone LO-MK-2

Immunogen :

purified IgG k from BALB/c mice

Reactivity :

k Light Chain of Mouse Immunoglobulin (determined by immunodot), positively tested on BALB/ andC57B1/7 mouse sera. LO-Mk-1 and LO-Mk-2 do not bind to the same epitope.

Cross-reactivity :

Does not cross-react with chicken, rabbit, goat, horse and baboon human IgG and with human IgM. Cross-react with dog, sheep, swine and bovine IgG (ELISA Test), probably by their k light chain, but not tested.

Avidity :

on mouse k IgM: 2 x 108 M-1; on mouse k IgG1: 1 x 109 M-1; on mouse k IgG2a: 3 x 109 M-1; on mouse k IgG2b: 2 x 109 M-1; on mouse k IgG3: 2 x 109 M-1

Applications :

Cf Reactivity
- Labelled second antibody, but less efficient than LO-Mk-1
- Can be labelled with Peroxidase, Fitc and Biotin
- Capture Elisa: good binding on plastics
- Can be used in Immunoaffinity chromatography for purification of Mouse k Ig (Solid phase Sepharose 4B CNBr act.)

Lyophilization :

Yes.

References

Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y.: 615-618 -

Selected References of scientific publications in which LO-Mk-2 Mab was used: Delaunay T. et al. J. Immunol. Methods, 1990, 131: 33-39. Vanhove B., Bazin H. Immunology 1993, 79: 580-586.

Rat IgMk - clone LO-MK-3

Immunogen :

purified IgG k from BALB/c mice

Reactivity :

k Light Chain of Mouse Immunoglobulin (determined by immunodot).

Cross-reactivity :

Doesnít cross-react with rat Ig (ELISA test).

Avidity :

on mouse IgG2a k: 1 x 107 M-1. Cf. Avidity sheet, for more details

Applications :

Cf Reactivity
- capture Elisa: low binding on plastics

Availability :

Only in ascitic fluid or supernatant forms.

Lyophilization :

Yes, but with denaturation.

References

Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids 1982, Peeters E. ed., 29th Colloquium 1981, Pergamon Press, Oxford and N.Y., pp. 615- 618.

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