Gene-PAGE - Code X309, X388, K117 or J228

Directions for use

For best results, this product should be allowed to warm to room temperature before use.

 

Note: On occasion, the Urea used as a denaturant in Gene-PAGE may begin to re-crystallize when stored at the recommended 4°C. This will not affect product performance, as long as the Urea is returned to solution before use. Simply place the product in a 37°C water bath for ten minutes, swirl the product briefly to re-mix, and use as directed below.

1- Determine the total volume of the gel plates you are using. Aliquot the required amount of Gene-PAGE into a sidearm flask.

Note: It is usually helpful to allow an additional 10 ml to accommodate any possible leaks in the apparatus.). Degas under gentle vacuum for five to ten minutes at room temperature.

2- Add Ammonium Persulfate (code 0486) from a 10% stock solution freshly prepared in deionized water at a ratio of 100 µL for each 10 ml of Gene-PAGE, and TEMED (code 0761) at a ratio of 10 L for each 10 ml Gene-PAGE. If slower polymerization is desired, 60 µL of 10% ammonium persulfate solution and 6 µL of TEMED may be used, respectively. Mix the solutions by swirling gently, and immediately pour into a prepared glass plate assembly. Insert an appropriately sized comb into assembly and allow gel to polymerize completely (30-45 minutes in most cases).

Gene-PAGE is intended for use with 1X TBE buffer. TBE is available from AMRESCO in a 10X liquid concentrate (code 0658) and a 10X Ready-Pack( (code 0478).

 

Also Available from AMRESCO:

  • Acryl/Bis 19:1, 29:1, 37.5:1 (30:0.8) solutions and pre-mixed powders.

  • Acryl-40

  • Acrylamide

  • Bis-2

  • bis-Acrylamide

  • TG-SDS, TG, TBE, TAE buffers in solution, pre-mixed powders, or Ready-Pack forms.

  • Acryl-Glide (Silane replacement)

Montluçon

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03103 Montluçon cedex - France

tel (33) (0) 4 70 03 88 55 

fax(33)(0) 4 70 03 82 60

interbiotech@interchim.com

 http://www.interchim.com

Vos contacts:

Etienne Boireau - Olivier Cadas - Olivier Alméras

hot line  33 (0) 4 70 03 73 06