Gene-PAGE PLUS - 4% E565,6% E568,8% E571

Directions for use

Note: On occasion, the Urea used as a denaturant in Gene-PAGE PLUS may begin to re-crystallize when stored at the recommended 4°C. This will not affect product performance as long as the Urea is returned to solution before use. Simply place the product in a 37°C water bath for ten minutes, swirl the product briefly to re-mix, and use as directed below.

• Determine the total volume of the gel plates you are using. Aliquot the required amount of Gene-PAGE PLUS into a sidearm flask. (Note: It is usually helpful to allow an additional 10 ml to accommodate any possible leaks in the apparatus.) De-gas under gentle vacuum for five to ten minutes at room temperature.

• Add Ammonium Persulfate (code 0486) from a 10% stock solution freshly prepared in deionized water at a ratio of 100 L for each 10 ml of Gene-PAGE PLUS and TEMED (code 0761) at a ratio of 10 L for each 10 ml Gene-PAGE PLUS. Please note that polymerization with these amounts will result in <10 minutes. If slower polymerization is desired, 60 L of 10% ammonium persulfate solution and 6 L of TEMED may be used, respectively. Mix the solutions by swirling gently, and immediately pour into a prepared glass plate assembly. Insert an appropriately sized comb into assembly and allow gel to polymerize completely (30-45 minutes in most cases). Proceed with electrophoresis as usual.

Gene-PAGE PLUS is intended for use with 1X TBE buffer. TBE is available from AMRESCO in a 10X liquid concentrate (code 0658) and a 10X Ready-Pack( (code 0478).

• PAGE-PLUS Concentrate

• TBE, TAE buffers in solution, pre-mixed powders or Ready-Pack forms

• Acryl-Glide (Silane replacement)

Montluçon

211 Bis Avenue Kennedy - BP 1140

tel (33) (0) 4 70 03 88 55 

fax(33)(0) 4 70 03 82 60

 http://www.interchim.com

Vos contacts:

Etienne Boireau - Olivier Cadas - Olivier Alméras

hot line  33 (0) 4 70 03 73 06