-
Cut open package
and remove gels. Rinse gel plates with deionized water.
-
To remove
comb,
hold cassette by the sides with both hands and gently push up evenly on the comb with both
thumbs.
Rinse wells gently with deionized water and empty by blotting or
inverting and shaking vigorously.
-
Place the gel into the electrophoresis apparatus. The shorter plate should
face the inner cathode
buffer chamber in most equipment. If you are using any of the
electrophoresis units
listed here, certain modifications and the use of an adaptor plate are
required. If you
-
need
an adaptor plate and have not received one, please contact your AMRESCO
Sales Representative. Directions on how to implement the modifications are
provided with the adaptor
plate.
-
Systems
requiring adjustments:
-
Fill
the cathode chamber with running buffer. Buffer level should be higher than the shorter
glass
plate and buffer should fill the wells. If the buffer chamber is leaking,
disassemble and reassemble.
-
Add buffer to the anode chamber so that the level of buffer is at least
one inch (2cm) above the
electrode wire at the bottom of the chamber. Be sure that any air bubbles
trapped under the lower
edge of the gel are released.
-
Load samples (up to 25 µL). Note: Samples should be prepared with a
standard sample buffer
that contains glycerol or sucrose to provide density so that samples will
sink to the bottom of
the wells.
-
Attach the system to the power supply according to manufacturer
instructions and run gel appropriately.
-
Shut off power, disconnect electrodes and remove the gel from the
electrophoresis unit.
-
With the shorter, notched plate facing up, peel off tape along the right
side of cassette and carefully pry apart the glass plates. Run a blade
along both side spacers to loosen gel and gently
lift the gel from the glass. Rinse gel with deionized water and stain, fix
or transfer gel as required.
