Antibiotic and Antifongic Agents
Applications
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in vitro culture: For invitro culture of bacterial and animal cells, it is essential to remove undesired bacterial and fungal strains that were present in the inoculum, or come by contamination.
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cultures vectors (recombinant protein production)
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hybridoma (monoclonal antibody production): Most vectors, both DNA plasmides and phages, and hybridoma, contain genes encoding for antibiotic resistance. The resistance identifies the vectors, and conferthe resitance to the hosting cell (bacteria or mamalian cell), and permit the positive selection of transfected cells: antibiotic and antifungic agents block, or kill undesired organisme by several means, but don’t affect the cultivated cell.
Technical inforamtion
The culture media, prepared or purchased, are classically autoclaved, cooled to 50°C, then supplemented with antibiotic solutions.
Antibiotic solutions may too be applied by vaporisation directly onto agar petri dishes, or included in agar gel preparation.
All should be maintained at 4°C for a maximum time of 30 days to maintain their efficacy. Without further indication, antibiotics may be prepared in filter-sterilized distilled water, and stored frozen at –20°C.
| Designation | Cat.number | Qty |
| Ampicillin | UP391961 | 10 g |
| Carbenicillin, disodium salt | UP383880 | 1 g |
| Chloramphenicol | UP091421 | 50 g |
| Kanamycin sulphate | UP308664 | 5 g |
| Rifampicin | UP375578 | 1 g |
| Streptomycin sulphate | UP224968 | 100 g |
| Tetracyclin hydrochloride | UP259169 | 50 g |
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All these reagent are produced according state of art procedures to ensure optimal results in cell culture |
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