Montluçon
211 Bis Avenue Kennedy
BP 1140
03103 Montluçon cedex - France
tel (33) (0) 4 70 03 88 55
fax(33)(0) 4 70 03 82 60
http://www.interchim.com
Vos contacts:
Etienne Boireau - Olivier Cadas - Olivier Alméras
hot line 33 (0) 4 70 03 73 06
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CleanSweep DNA Clean-up Kit (E686) For removing linkers and primers from enzymatic reactions Directions for Use |
| System Components: |
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CS-1 5 mg **/10 labels CS-2 1 ml CS-3 10 ml CS-4 25 ml ** Upon arrival, CS-1 should be reconstituted in 1 ml sterile dH2O, separated into aliquots and labeled (labels provided) before storing at -20°C. |
| Storage: |
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CS-1 Frozen CS-2 Room Temperature CS-3 Room Temperature CS-4 Room Temperature |
| INSTRUCTIONS: |
| Step 1: Following enzymatic reaction, adjust the volume of the reaction solution to 100 L with dH2O. |
| Step 2:
Add 5 L of CS-1, 10
L of CS-2 and 60 L of CS-3. Vortex to mix. Centrifuge at high speed in a
microcentrifuge at room temperature for 5 minutes.
* For small linkers (< 30bp), step 3-5 may be eliminated * |
| Step 3: Discard the supernatant. |
| Step 4: To the pellet, add 100 L of H2O. Vortex to dissolve pellet. |
| Step 5: Add 60 L of CS-4. Vortex to mix. Centrifuge at high speed in a microcentrifuge at room temperature for 4 minutes. |
| Step 6:
Carefully remove
the supernatant and add to the pellet 200 l of CS-5.
Vortex to mix. Centrifuge at high speed in a microcentrifuge at room temperature for 1 minute. Remove the supernatant and dry the pellet under vacuum. |
| Step 7: The
sample can now be dissolved in the desired volume of dH2O
or TE.
For Hazards & Additional Information see MSDS. |
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Montluçon 211 Bis Avenue Kennedy BP 1140 03103 Montluçon cedex - France tel (33) (0) 4 70 03 88 55 fax(33)(0) 4 70 03 82 60 http://www.interchim.com |
Vos contacts: Etienne Boireau - Olivier Cadas - Olivier Alméras hot line 33 (0) 4 70 03 73 06 |
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