Primer and Cover Oil Remover For removing primers and mineral oil from PCR* samples

Directions for Use

S-1 5 mg **/10 labels

S-2 1 ml

S-3 50 ml

S-4 10 ml

S-5 25 ml

** Upon arrival, S-1 should be reconstituted in 1 ml sterile diH2O, separated into aliquots and labeled (labels provided) before storing at -20°C.

S-1 Frozen

S-2 Room Temperature

S-3 Room Temperature

S-4 Room Temperature

S-5 Room Temperature

Step 1: Following PCR reactions, transfer the reaction solution (lower phase) into a new 1.5 ml microcentrifuge tube. Care should be taken not to transfer large volumes of the mineral oil layer. Adjust the volume of the reaction solution to 100 L with diH2O.

Step 2: Add 5 L of S-1, 10 L of S-2 and 200 L of S-3. Vortex to mix. Centrifuge at high speed in a microcentrifuge at room temperature for 5 minutes.

Step 3: Discard the supernatant. Re-suspend the pellet in 200 L of S-3. Vortex to mix. Centrifuge at high speed in a microcentrifuge at room temperature for 1 minute.

Step 4: Discard the supernatant.

Step 5: To the pellet, add 100 l of diH2O. Vortex to dissolve pellet.

Step 6: Add 60 L of S-4. Vortex to mix. Centrifuge at high speed in a microcentrifuge at room temperature for 4 minutes.

Step 7: Carefully remove the supernatant and add to the pellet, 200 L of S-5.

Vortex to mix. Centrifuge at high speed in a microcentrifuge at room temperature for 1 minute. Remove the supernatant and dry the pellet under vacuum.

Step 8: The sample can now be dissolved in the desired volume of diH2O or TE.

For Hazards & Additional Information see MSDS.

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