Rapid RNA

Rapid RNA Purification Kit Short Protocols

Directions for Use

(Important : Read complete Directions for Use and all Application Notes before attempting this procedure)

To extract RNA from Tissues:	To extract RNA from Cells:
To 100 mg tissue, add 500 L Reagent 1. add 50 L Tissue Homogenization Suspension Homogenize tissue thoroughly	Wash cell monolayer 2X in ice-cold PBS buffer Add 600 L Reagent 1. Add 100 L Reagent 3. Incubate 5 minutes at Room Temp
Centrifuge 2 minutes Room Temp.	Scrape cells and transfer to a sterile centrifuge tube. Incubate 5-10 minutes on ice
Transfer supernatant to a fresh centrifuge tube. Add 500 L Reagent 2.Vortex thoroughly to mix.	Add 300 L Reagent 4. Incubate 5 minutes on ice
Centrifuge 3 minutes Room Temp.	Centrifuge 5 minutes at Room Temp
Transfer the aqueous layer to a fresh tube. Add 100 L Reagent 3. Vortex to mix. Add 300 L Reagent 4. Vortex to mix. Incubate on ice 3 minutes.	Transfer supernatant to a fresh tube Add 1 mL Reagent 2. Vortex thoroughly to mix.
Centrifuge 2 minutes Room Temp.	Centrifuge 3 minutes at Room Temp
Transfer supernatant to a fresh tube. Add 600 L RNA Precipitation Reagent. Mix thoroughly by inversion	Transfer aqueous layer to a fresh tube Add 600 L RNA Precipitation Reagent. Mix thoroughly by inversion.
Centrifuge 5 minutes Room Temp to pellet RNA.	Centrifuge 5 minutes at Room Temp to pellet RNA.
Wash pellet twice with 70% Ethanol	Wash pellet twice with 70% Ethanol.

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