Protein Assays - BC Assay
High quality and otpimized formulated reagents
| Biochemistry of Proteins |
Protein Assays - BC Assay High quality and otpimized formulated reagents |
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| Protein Assays introduction | BC Assay | Micro BC assay | Coo Assay |
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BC Assay for Protein quantitation |
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(modified lowry assay, based on bicinchoninic acid) A very versatile and efficient assay, suitable for most applications. Detergents compatible. | |||
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Protocol |
Standard Assay |
Room Temperature |
Enhanced | ||
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Reagent |
Mix 2 components A+B (50:1) | ||||
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Sample (in tube microplate) |
100µl (25µl) |
100µl (25µl) |
100µl (25µl) | ||
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Reagent |
2 ml (200µl) |
2 ml (200µl) |
2 ml (200µl) | ||
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Incubation |
30min at 37°C |
2h at Room Temperature |
30mn at 60°C | ||
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OD reading |
562nm (540-590nm) | ||||
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Comments |
Flexible protocols (temperature and duration) - (One minute protocole with microwave heating) - Great accuracy | ||||
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Performances / features Overcomes often Coomassie assay | |||||
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Accuracy,Linearity |
Very good, 20-1000µg/ml | ||||
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Protein to protein variations |
Low (3-4 fold lower than with the Coo Assay) | ||||
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Sensitivity and working range |
20-2000µg/ml |
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5-500µg/ml | ||
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Stability |
>1 year at Room Temperature | ||||
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Compatibility |
Most detergents, chaotropic agents, preservatives (anti-microbials…), inhibitors (of proteases…), buffer and salts | ||||
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Incompatibility |
Reducing agents (sugars, thiols…), copper chelators, cys, tyr, try amino-acids | ||||
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Applications | |||||
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Samples |
Purified proteins, complex mixtures, polypeptides, immobilized proteins... Notably containing: nucleic acids, lipids, detergents… Alkalin samples | ||||
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Others |
Determination of Copper concentration | ||||
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Catalog Number |
Description | |
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BC Assay - protein Quantitation Kit, complete kit for 500 (tube) or 5000 (microplate) determinations | ||
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Contains: |
reagent A, 1 L | |
| reagent B, 25 ml | ||
| - | Serum Bovine Albumine Standard, 2 mg/ml, 10x1ml | |
| BC Assay - protein Quantitation Kit, complete small kit for 125 (tube) or 1250 (microplate) determinations | ||
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Contains: |
reagent A, 250 ml | |
| reagent B, 6 ml | ||
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UP36859A, |
Serum Bovine Albumine Standard, 2mg/ml, 3x1ml |
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211 Bis Avenue Kennedy - BP 1140 - 03103 Montluçon cedex - France - Tel (33) (0) 4 70 03 88 55 - Fax (33) (0) 4 70 03 82 60 |
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