RNA Study

Molecular Biology

RNA study

UptiRNAPure Total RNA Purification Kit - from animal tissues and cellular cultures
UptiReverse cDNA/DNA Polymerase
Nucleotides:
ATP, Na₄ - GTP, Na₄ - CTP, Na₄ - UTP, Na₄ - NTP, Set
Related Product: MOPS

UptiDNAPure Total RNA Purification Kit - from animal tissues and cellular cultures

Applications

Total RNA Purification from animal tissues and cell cultures.
Purified RNA can be used in most downstream applications (reverse transcription-amplification, labeling, Northern blotting, etc.).
No phenolic extractions. Based on a modified Silica matrix, that binds RNA with a high specificity.
High integrity and purity of obtained RNA, without DNA contamination.
Fast and easy method: protocol completed in 35 minutes.

Description	Cat.#	Qty
UptiDNAPure Total RNA Purification Kit from animal tissues and cellular cultures	UPS54671	25rxns
	UPS54672	50rxns
--	UPS54673	100rxns

See also: Phenol, saturated

Quality control

Each lot is thoroughly tested to ensure maximum quality and lot to lot reproducibility. A total of 30 preparations are processed and used in a variety of downstream applications.

Store at 4ºC or room temperature (depending on reagent).

Figure1.

Total RNA from 320 mg of rat liver obtained using UptiRNAPure Total RNA Purification Kit from animal tissues and cellular cultures.

RNA was eluted in a final volume of 100 µl. M: 100bp ladder Marker. Lane1: 1 µl of final eluate. Lane 2: 1/5 dilution of lane1. Lane 3: 1/5 dilution of lane 2.

Nucleotides

ATP, Na₄	UP299232	25µmol
Adenosine-5’-triphosphate, tetrasodium salt, 100mM
CAS [987-65-5] C₁₀H₁₂N₅O₁₄P₃Na₄ MW 595.15 Absorbance max. : 259 nm e at absorbance max : 9,0 E x mmol^-1 x cm^-1 pH 7.0 Purity > 98 % ATP is suitable for in vitro transcription and other in vitro assays. ATP is used as substrate for the reduction reaction to form the dATP. ATP is also used as energy source of enzymatic reactions.
CTP, Na₄	UP314781	25µmol
Cytidine-5’-triphosphate, tetrasodium salt, 100mM
CAS [81012-87-5] C₉H₁₂N₃O₁₃P₃Na₄ MW 571.13 Absorbance max. : 271 nm e at absorbance max : 9,0 E x mmol^-1 x cm^-1 pH 7.0 Purity > 98 % CTP is used as substrate for the reduction reaction to form dCTP. CTP is suitable for in vitro RNA transcription.
NTP, Set	UP968680	4x25µmol
	UP968681	4x25µmol
Ultrapure NTP Set, Nucleoside 5’-triphosphates, tetrasodium salts (100mM) Purity > 98 % Store at –20°C NTPs are used as substrates for the reduction reaction to form dNTPs_(1,2). NTPs are suitable for in vitro RNA transcription Literature : (1) Vitols, E., Blakley,R.L., J.Biol.Chem.,242,3035 (1967) (2) Blakley, R.L, Methods in enzymology, Vol.II,246 (1978)

GTP, Na₄	UP367852		25µmol
Guanosine-5’-triphosphate, tetrasodium salt, 100mM
CAS [36051-31-7] C₁₀H₁₄N₅O₁₄P₃Na₄ MW 611.15 Absorbance max. : 253 nm e at absorbance max : 9,0 E x mmol^-1 x cm^-1 pH 7.0 Purity > 98 % GTP is used as substrate for the reduction reaction to form dGTP. GTP is RNAse free so is suitable for in vitro RNA transcription. GTP acts as coenzyme in the cell free protein synthesis.
UTP, Na₄	UP23636A		25µmol
Uridine-5’-triphosphate, tetrasodium salt, 100mM
CAS [19817-92-6] C₉H₁₁N₂O₁₅P₃Na₄ MW 572.10 Absorbance max. : 262 nm e at absorbance max : 9,0 E x mmol^-1 x cm^-1 pH 7.0 Purity > 98 % UTP is used as substrate for the reduction reaction to form dUTP. UTP is suitable for in vitro RNA transcription.



Related Products
MOPS		UP06200
These buffer is commonly used for nucleotide solubilisation

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