Biochemistry of Proteins

Protein Assays - coo Assay

 High quality and otpimized formulated reagents

 

 

Protein Assays introduction BC Assay Micro BC assay Coo Assay

 

Coo Assay for Protein quantitation

Technical Sheet

Technical Notice: comparison 

 Securityl Sheet

(modified Bradford assay, based on Coomassie dye)

A very popular assay, worldwide known and documented. Reducing agent compatible.

 

Protocol

Broad range

Intermediate

High Sensitivity

Max Sensitiivity

Reagent

1 ready-to-use reagent (Coo)

1 ready-to-use reagent (Coo)

Sample in tube (microplate) 

40µl (5µl)

50µl (10µl)

200µl (25µl)

1ml (150µl)

Reagent

2ml (250µl)

1.5ml (300µl)

2ml (250µl)

1ml (150µl)

Incubation

1min at Room Temperature

OD reading

595nm (570-610nn)

Comments

Very easy and quick. No filtration needed! Corrosive

Performances / features     Overcomes often Coomassie assay

Accuracy, Linearity

50-1500µg/ml

50-800µg/ml

20-200µg/ml

1-25µg/ml

Protein to protein variations

Noticable

Noticable

Sensitivity and working range

50-2000µg/ml

50-1000µg/ml

20-500µg/ml

1-100µg/ml

Stability

1 year at 4°C

Compatibility

Reducing agents (80mM DTT…), 2% Triton X100, 400mM Bicarbonate…

Incompatibility

Incompatible with numerous detergents, strong Alkaline solutions

Applications

Samples

Purified proteins, complex mixtures

Acidic samples

Reducing agents containing samples

 

 

 

 

 

 

Catalog Number

Description

UPF86400

Coomassie Protein Assay - complete classic kit for 500 (tube) or 4000 (microplate) determinations

Contains:

UPF86420, 

Coomassie reagent, 1L

-

UP36859A, 

 Bovine albumin standard, 2mg/ml, 10x1ml

UP75860C

Coomassie Protein Assay - complete small kit for 125 (tube) or 1000 (microplate) determinations

Contains:

UPF86421

Coomassie reagent, 250ml

-

UP36859A

 Bovine albumin standard, 2mg/ml, 3x1ml

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