Electrophoresis Gel Matrix

Molecular Biology

electrophoresis gel matrix

Acrylamides solutions

Acrylamides, Solution 4X-40% - Bis-Acrylamide, Solution 2% - Acrylamides/Bis-Acrylamide 19:1, Solution 40% -

Acrylamides/Bis-Acrylamide 29:1, Solution 40% - Acrylamides/Bis-Acrylamide 37.5:1, Solution 40%
Other biochemicals for electrophoresis

Ammonium Persuflate - TEMED - Formamide, deionized - SDS, powder - SDS, 20% solution - Urea
Agaroses

Agarose MB - Agarose HR - Agarose HR0.7
Related product:

Ethidium Bromide

Acrylamides solutions

Ready to use 40% solution.
Prepared with the highest quality products : 4X acrylamide, 2X bis-acrylamide.
No hazard powder.

Liquid Acrylamide products were designed to replace powdered materials with convenient, "user friendly" solutions. These solutions are provided in a ready-to-use format, thereby eliminating inhalation hazards associated with handling acrylamide and bis-acrylamide powders.

Liquid Acrylamides produce highly reproducible gels with excellent band mobility and resolution. Concentrations are carefully controlled for maximum lot-to-lot consistency and all solutions are 0.2 um micro-filtered prior to packaging.

Liquid Acrylamide products are prepared from Ultra Pure Grade components in specially treated, deionized water and are guaranteed stable for at least one year when properly stored.

Acrylamide/Bis-Acrylamide 19:1

is a 40% solution containing 38% (w/v) acrylamide and 2% (w/v) bis-acrylamide. Optimized for nucleic acid electrophoresis. Final ratio of monomer to cross-linker is 19:1.

Acrylamide/Bis-Acrylamide 29:1

is a 40% solution containing 38.67% (w/v) acrylamide and 1.33% (w/v) bis-acrylamide. Excellent for many protein electrophoresis applications and can also be used for nucleic acid electrophoresis. Final monomer to cross-linker ratio is 29:1.

Acrylamide/Bis-Acrylamide 37.5:1 (30:0.8)

is a solution of 38.96% (w/v) acrylamide and 1.04% (w/v) bis-acrylamide. Optimized for most protein separations. Final ratio is 37.5:1 (30:0.8).

Security Sheet

Acrylamides	UP873376	500ml
Solution 4X-40%	UP873377	2x500ml
Bis-Acrylamide	UP864965	500ml
Solution 2%	UP864966	2x500ml
Acrylamides/Bis-Acrylamide 19:1	UP86489B	500ml
Solution 40%	UP86489C	2x500ml
Acrylamides/Bis-Acrylamide 29:1	UP864927	500ml
Solution 40%	UP864928	2x500ml
Acrylamides/Bis-Acrylamide 37.5:1	UP864937	500ml
Solution 40%	UP864938	2x500ml

Other biochemicals for electrophoresis

Ammonium Persuflate	UP306098	4x25g
ACS Grade -
(NH₄)₂S₂O₈ CAS [7727-57-0] MW 228.20 Purity 98.0 % Titrable Free Acid < 0.04 meq/g Storage at store at room temperature
Formamide, deionized		UP07099

TEMED	UP15413D	25ml
(N,N,N’,N’-TETRAMETHYLETHYLENEDIAMINE) Ultra pure grade
C₆H₁₆N₂ CAS [10-18-9] MW 116.21 Purity (Anhydrous Basis) : 99.0 % Storage at store at room temperature. -
SDS, powder	UP64910
SDS, 20% solution	UP89682
Urea

Agaroses

Agarose MB	UPS54180	100g
	UPS54181	250g
for analytical electrophoresis in Molecular Biology

Applications

Agarose UPTIMA has been specially designed for analytical electrophoresis in routine Molecular Biology analysis. Separation range is 0.2 - 22 Kbp, depending on agarose concentration and buffer used for electrophoresis.

Fig1.

Four samples were amplified using Cytochrome b specific primers (lanes 1-4), using Uptima UptiTherm DNA Polymerase (Cat.No. UPS5 3661).

The 358 bp product was separated by electrophoresis using Uptima agarose (1.5% gel) in TBE buffer for 35 min (5V/cm). The Uptima agarose shows an exceptional resolution as well as clarity and performance, even for the visualisation of 100 bp molecular weight marker (Cat.No. UPS54811).

Technical Information

Appearance	White fine powder
Gel strength (1%)	1200 gr / cm2
Gelling range (1%)	36-39ºC
Melting range	87 - 89 ºC
EEO (relative mobility) (1%)	< 0.10
Sulphates	< 0 - 10 %
DNase, Rnase	Non detected
Protease activities	Non detected

Quality control

Each lot is thoroughly controled to ensure maximum performance and lot to lot reproducibility.

Store at room temperature.

Agarose HR	UPS54200	50g
	UPS54201	100g
for high resolutionl electrophoresis

Applications

Agarose UPTIMA has been specially designed for analytical electrophoresis in routine Molecular Biology analysis. Separation range is 20 - 2000bp, depending on agarose concentration and buffer used for electrophoresis.

Fig 2.

Four samples were amplified using cytochrome b specific primers (lanes 1-4), using Uptima DNA Polymerase (Cat.No. UPS53661). The 358bp product was separated by electrophoresis using Uptima HR agarose 1.5% in TBE buffer for 35 min(5V/cm2).

The Uptima agarose HR shows an exceptional resolution as well as clarity and performance, even for the visualisation of 100 bp molecular weight marker (UPS54811).

Technical Information

Appearance	White fine powder
Gel strength (1%)	2000 gr / cm2
Gelling range (1%)	36-38ºC
Melting range	87 - 89 ºC
EEO (relative mobility) (1%)	< 0.15
Sulphates	< 0 - 15 %
DNase, Rnase	Non detected
Protease activities	Non detected

Quality control

Each lot is thoroughly controled to ensure maximum performance and lot to lot reproducibility.

Store at room temperature.

Agarose HR0.7	UPS54252	1L
	UPS54253	2L
for high resolution electrophoresis using 0.7% standard agarose

Applications

Uptima Agarose HR0.7 (0.7% standard agarose) substitutes high-resolution agaroses for the discrimination of DNA fragments <500bp.

Revolutionary: up to 6 - 10 bp discrimination.

Savvy: cuts down agarose costs.

DNA can be purified from the Agarose HR0.7 Matrix using standard methods.

Fast and easy to use.

Agarose HR0.7 Matrix kit includes Uptima MB Agarose.

Quality control

Each lot is thoroughly controled to ensure maximum performance and lot to lot reproducibility.

Store at <25°C

Fig 3.

Agarose HR0.7 gel showing duplicate samples of three molecular ladders : FX174-Hinfl (lanes 1-2, Cat. UPS54781), pBR322/BsuRI (3-4, Cat. UPS54791), and 100 bp-ladder (5-6, Cat.UPS54811). Picture to the left depicts low-molecular weight bands with sharper contrast.

Related product

Ethidium Bromide	UP89244B	10ml
10mg/ml solution
C₂₁H₂0BrN₃ CAS [1239-45-8] MW 394,32
Ethidium Bromide solution is the safer than powder and most convenient means to prepare gels, solutions and gradients for your molecular biology applications. Ethidium bromide intercalates double-stranded DNA and RNA2. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 mg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids^3-5. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA⁶.

Ethidium Bromide is a nucleic acid intercalating agen and frameshift mutagen1. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells3.

Literature :

1- Scaria, P.V. , Shafer, R.H., Binding of ethidium bromide to a DNA triple helix. Evidence for intercalation J. Biol. Chem. 266, 5417 (1991)

2- Sambrook, J., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, (1989), 6-44, 6

3- Karsten, U. , Wollenberger, A., Improvements in the ethidium bromide method for direct fluorometric estimation of DNA and RNA in cell and tissue homogenates Anal. Biochem. 77, 464 (1977)

4- El-Hamalawi, A., The fluorometric determination of nucleic acids in pea seeds by use of ethidium bromide complexes. Anal. Biochem. 67, 384 (1975)

5- Moore, S.P. , Sutherland, B.M., A densitometric nondestructive microassay for DNA quantitation Anal. Biochem. 144, 15 (1985)

6- Severini, A. , Morgan, A.R., An assay for proteinases and their inhibitors based on DNA/ethidium bromide fluorescence Anal. Biochem. 193, 83 (1991)

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