Biochemistry of Proteins

IMMUNIZATION

MaxiBind™ Super Active Carriers!

 

 

Carriers (KLH) MaxiBind TiterMax Hybridokine

 

MaxiBindTM  proteins offer a growing number of amine functions to participate to coupling reactions with haptens or macromolecules and to improve the presentation of conjugates to the immune system.

These proteins are therefore proving to be the best choice for many applications for the preparation of conjugates and particularly to obtain very immunogenic carriers-haptens which can be used for immunization tests.

 

Specifications

Applications

MaxiBindTM proteins are altered chemically on carbolyxic functions by diamine ethylene.

This alteration introduces ammoniums hiding negative charges of carboxylates and resulting in a significant increase of the Isoelectric Point (PI) [1]

The result is a "cationized" molecule essentially positive and reactive charges.

  • Reactivity on :

    • aldehydes

    • carboxylic acids

  • Optimal number of reactive amines

    • conjugates at a high rate of couplings

  • Positive ionic charge :

    • easy internalization in immunocompetent cells

  • High purity

  • Batch to batch (CV <10%) :

    • from R&D to manufacturing

    • easy scale changes

  • Stability

Production of antibodies

Immunogenic conjugates :

MaxiBind™ carriers (usually BSA) improves and increases the quantity and quality of the immune response [1] [2] [3] [4]

  • Faster induction of the immune response (both cellular and humoral)

  • Higher quantity of specific induced antibodies

  • Improved affinity of antibodies

Conjugated antigens for tests :

MaxiBind™ carriers track better your specific antibodies !

  • Ideal for ELISA coating to monitore immunization

  • Useful as tracer or inhibitor in competitive assays

  • Double screening : 2 or 3 carriers allow to analyse cross-reactivity toward carrier and linker ; notably useful for monoclonal antibodies.

Studies of ligand interactions

  • Pharmacology : drug-carriers

  • Pharmacocinetics (distribution, elimination..) : tracers

  • Studies of receptors : cell binding

  • Studies of the endocytose

  • Biocaptors : cationized surfaces, special coatings

BSA MaxiBindTM

UP84225A

2mg

OVA MaxiBindTM

UP98505A

2mg

Cationized Bovine Albumin 68-69 kDa

UP84225B

5mg

Cationized Ovalbumin 44-45 kDa

UP98505B

5mg

UP84225C

10mg

UP84225D

50mg

Main use:

Carriers / Immunogens

Conjugates for various studies

Ideal to prepare your immunogens for the production of antibodies, especially against little immunogenic haptens, peptides,…[1] :

  • Faster induction of the immune response ( cellular or humoral )

  • Higher quantity of specific induced antibodies

  • Improved affinity of antibodies

Main use:

Carriers / Immunogens

Immunization tests

Conjugates for various studies

A classic for your immunization test conjugates:

  • Easy bind on to activated or non activated stands and in particular on to ELISA microplaques (screening of monoclonal antibodies, immunization follow up)

  • Creates conjugates at a high hapten/protein coupling rate allowing the detection of weak concentrations ( IgM, IgG…)

Æ A 100 % increase of the number of reactive amine fonctions against the native BSA (70 functions instead of 35).

Æ Ideal for light weight molecular haptens or for haptens wich are difficult to coat

Æ High detection sensitivity

b-LG MaxiBindTM

UP98506A

2mg

 

 

Cationized b-LactoGlobulin

UP98506B

5mg

UP98506C

10mg

UP98506D

50mg

Main use:

Immunization tests

Conjugates for various studies

Ideal to prepare your immunogens for the production of antibodies, especially against little immunogenic haptens, peptides,…[1] :

  • The background are the lowest for some detection systems.

  • Can be used for immunization when one fears the induction of antibodies with an hapten/albumin complex (ionic or hydrophobes link)

Æ A carrier protein which is little known but which is very interesting

Æ Ideal for the most applications demanding high specificity (double screening of monoclonal antibodies).
Principle of coupling

Protocol

Technical Sheet

Coupling MaxiBind to COOH bearing molecules is easy !

  • Dissolve the hapten in a minimum amount of coupling buffer

  • Add EDC( #UP52005), and NHS (#UP04594)

  • Add Maxibind™ protein directly into the solution.

  • Stir at room temperature overnight.

  • Purify the hapten-carrier conjugate

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