Protein Assays - Micro BC Assay
High quality and otpimized formulated reagents
| Biochemistry of Proteins |
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Protein Assays - Micro BC Assay High quality and otpimized formulated reagents |
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| Protein Assays introduction | BC Assay | Micro BC assay | Coo Assay |
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Micro BC Assay, for Protein Quantitation |
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(modified lowry assay, based on bicinchoninic acid) The high sensitivity version of the BC Assay |
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Protocol |
Standard Assay |
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Reagent |
mix 3 components A+B+C (25:25:1) |
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Sample (in tube microplate) |
1ml (150µl) |
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Reagent |
1ml (150µl) |
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Incubation |
1h at 37°C |
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OD reading |
562nm (540-590nm) |
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Performances / features Overcomes often Coomassie assay |
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Accuracy, Linearity |
Very good, 1-100µg/ml |
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Protein to protein variations |
Low (slightly better than BC Assay) |
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Sensitivity and working range |
1-200µg/ml |
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Stability |
>1 year at Room Temperature |
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Compatibility |
Similar to BC Assay, but compatible concentrations are slighly lower because of sample/reagent volume ratio |
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Incompatibility |
Same as for the BC Assay |
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Applications |
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Samples |
Purified proteins, complex mixtures, polypeptides... Notably containing: nucleic acids, lipids, detergents… Alkalin samples |
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Catalog Number |
Description |
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Micro BC Assay - protein Quantitation Kit, complete kit for 500 (tube) or 3400 (microplate) determinations |
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Contains : |
reagent A, 250ml |
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| reagent B, 250ml | ||
| reagent C, 12ml | ||
| - | BSA Standard, 10x1ml | |
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Micro BC Assay - protein Quantitation Kit, complete small kit for 50 (tube) or 340 (microplate) determinations |
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Contains : |
reagent A, 25ml |
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| reagent B, 25ml | ||
| UP67253C, | reagent C, 1.2ml | |
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UP36859A, |
BSA Standard, 1x1ml |
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211 Bis Avenue Kennedy - BP 1140 - 03103 Montluçon cedex - FRANCE Tel (33) (0) 4 70 03 88 55 - Fax (33) (0) 4 70 03 82 60 - uptima@interchim.com www.interchim.com |
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