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Blue-BANDit™ Ready-to-Use Protein Stain - Code K217-1L Product Information |
Coomassie® Blue staining of proteins in SDS-PAGE gels is a daily procedure in many laboratories. It is popular among life science researchers, due to its good sensitivity and relative ease of use. Traditionally, Coomassie Blue staining requires a methanol and acetic acid solution to achieve staining and destaining. This process increases the risk for hazardous exposure and produces an unpleasant pungent odor. AMRESCO’s Blue-BANDit is a convenient alternative to traditional Coomassie Blue staining procedures. Environmentally friendly, this ready-to-use stain does not contain methanol and acetic acid and does not require hazardous solvents for destaining. The simple ”hands-off” staining/destaining procedure saves valuable time while reducing the handling of hazardous materials and solvent waste in your laboratory. Blue-BANDit staining exhibits sensitivity below 20 ng of protein per band. Packaged as a 1X ready-to-use solution, Blue-BANDit only requires water for the prewashing and destaining steps. |
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Results of an 8% polyacrylamide gel loaded with serial dilutions of High Range Protein Marker (40 - 212 kDa) using 1X TG-SDS buffer and visualized with Blue-BANDit (GelA) and Standard Coomassie Blue G-250 (Gel B). |
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| Blue-BANDit Staining Procedure: |
| 15 min | Pre-Wash with Deionized Water (for denaturing gels) |
| 1 hour | Staining with Blue-BANDit™ (completely submerge thegel) |
| 30 min | Destain in Deionized Water (may continue overnight) |
| AMRESCO provides an extensive line of acrylamides and high quality buffers to compliment your analytical applications in the area of protein research. | |
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211 Bis Avenue Kennedy - BP 114003103 Montluçon cedex - France tel (33) (0) 4 70 03 88 55 fax (33) (0) 4 70 03 82 60 http://www.interchim.com |
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