Blue-BANDit Ready-to-Use Protein Stain - Code K217-1L

Product Information


  • Hands-Off! Simple Staining/Destaining Procedure

  • High Sensitivity, Below 20 ng of Protein per Band

  • Safer Alternative, No More Methanol and Acetic Acid

Coomassie® Blue staining of proteins in SDS-PAGE gels is a daily procedure in many laboratories.  It is popular among life science researchers, due to its good sensitivity and relative ease of use.  Traditionally, Coomassie Blue staining requires a methanol and acetic acid solution to achieve staining and destaining.  This process increases the risk for hazardous exposure and produces an unpleasant pungent odor.

AMRESCO’s Blue-BANDit is a convenient alternative to traditional Coomassie Blue staining procedures.  Environmentally friendly, this ready-to-use stain does not contain methanol and acetic acid and does not require hazardous solvents for destaining. The simple ”hands-off” staining/destaining procedure saves valuable time while reducing the handling of hazardous materials and solvent waste in your laboratory.

Blue-BANDit staining exhibits sensitivity below 20 ng of protein per band.  Packaged as a 1X ready-to-use solution, Blue-BANDit only requires water for the prewashing and destaining steps.

 

Gel A

Results of an 8% polyacrylamide gel loaded with serial dilutions of High Range Protein Marker (40 - 212 kDa) using 1X TG-SDS buffer and visualized with Blue-BANDit (GelA) and Standard Coomassie Blue G-250 (Gel B).

Gel B

 

Blue-BANDit Staining Procedure:
15 min Pre-Wash with Deionized Water (for denaturing gels)
1 hour Staining with Blue-BANDit™ (completely submerge thegel)
30 min Destain in Deionized Water (may continue overnight)
AMRESCO provides an extensive line of acrylamides and high quality buffers to compliment your analytical applications in the area of protein research.

 

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