Protein molecular weight markers - Code J786, J450, J449

Directions for use

General

Those marker sets are mixes of purified proteins of known MW used as standards in determining the apparent MW of unknown proteins run on SDS-polyacrylamide gel electrophoresis (SDS-PAGE).  Each set has been blended to provide bands of equal intensity upon staining.  The markers are suspended in a buffer containing 0.0625M Tris-HC1, pH 6.8, 2% SDS, 0.01M Dithiothreitol, 0.0125% Bromphenol Blue, and 20% Glycerol (High and Low Range Sets) or 50% Glycerol (Mid-Range).

Protocol

Transfer the desired amount of markers to a clear microcentrifuge tube.  Heat this aliquot at 95(C for 5 minutes to completely denature the markers, then load them onto an SDS-PAGE gel.  Two to three microliters of the stock solution is usually sufficient for a clear banding pattern.  If the aliquot size is less than 10 (l it can be diluted 5-10X with the SDS loading buffer.  This will prevent evaporation loss during the heating process and may also improve comparison with the samples.The markers are loaded on the gel and run in the same fashion as the comparison samples.

Results

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