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Références
bibliographiques du Strep-Tag
A-
Articles d’intérêt général - sélection
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Skerra
A, Schmidt TGM, 2000: Meth. Enzymol. 326: 271-304. Use of the Strep-tag
and streptavidin for recombinant protein purification and detection.
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Skerra
A, Schmidt TGM, 1999: Biomolecular Engineering 16: 79-86. Applications
of a Peptide Ligand for streptavidin: the Strep-tag.
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Müller
HN, Schmidt TGM, 2000: in Kastner M (Ed.), Journal of Chromatography
Library - volume 61, Protein Liquid Chromatography, Elsevier, ISBN 0
444 50210 6, pp. 825-837. Simple and fast one-step purification of
recombinant proteins using the unique Strep-tag
technology.
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Schmidt
TGM, Skerra A, 2000: in Alberghina L (Ed.), Protein Engineering in
Industrial Biotechnlogy, Harwood Academic Publishers, ISBN:
90-5702-412-8, pp. 41-61. Protein Engineering for Affinity
Purification: the Strep-tag.
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Schmidt
TGM, Koepke J, Frank R, Skerra A, 1996: J. Mol. Biol. 255: 753-766.
Molecular interaction between the Strep-tag
affinity peptide and its cognate target streptavidin.
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Lamla
T, Mammeri K, Erdmann VA, 2001: Acta Biochim. Pol. 48: 453-465. The
cell-free protein biosynthesis-applications and analysis of the
system.
B-
Expression avec promoteur tet
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Skerra
A, 1994: Gene 151: 131-135. Use of the tetracycline promoter for the
tightly regulated production of a murine antibody fragment in
Escherichia coli.
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Loferer
H, Hammar M, Normark S, 1997: Molecular Microbiology 26: 11-23.
Availability of the fibre subunit CsgA and the nucleator protein CsgB
during assembly of fibronectin-binding curli is limited by the
intracellular concentration of the novel lipoprotein CsgG.
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Korpela
MT, Kurittu JS, Karvinen JT, Karp MT, 1998: Anal. Chem. 70: 4457-4462.
A recombinant Escherichia coli sensor strain for the detection of
tetracyclines.
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Rau
D, Kramer K, Hock B, 2002: J Immunoassay Immunochem 23:129-43.
Single-chain Fv antibody-alkaline phosphatase fusion proteins produced
by one-step cloning as rapid detection tools for ELISA.
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Rau
D, Kramer K, Hock B, 2002: Anal. Bioanal. Chem. 372: 261-267. Cloning,
functional expression and kinetic characterization of
pesticide-selective Fab fragment variants derived by molecular
evolution of variable antibody genes.
C-
Purification
Après
expression dans cellules mammifères
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Ahrens,
T., Lambert, M., Pertz, O., Sasaki, T., Schulthess, T., Mège, R.-M.,
Timpl, R. and Engel, J. (2003). Homoassociation of VE-cadherin follows
a mechanism common to `classical` cadherins, J Mol Biol, 325.
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Ahrens,
T., Pertz, O., Häussinger, D., Fauser, C., Schulthess, T., and Engel,
J. (2002). Analysis of heterophilic and homophilic interactions of
cadherins using the c-Jun/c-Fos dimerization domains, J Biol Chem 277,
19455-19460.
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Sardy
M, Odenthal U, Karpati S, Paulsson M, Smyth N, 1999: Clin. Chem. 45:
2142-2149. Recombinant human tissue transglutaminase ELISA for the
diagnosis of gluten sensitive enteropathy.
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Smyth
N, Odenthal U, Merkl B, Paulsson M, 2000: Methods Mol. Biol.139:
49-57. Eukaryotic expression and purification of recombinant
extracellular matrix proteins carrying the Strep II tag.
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Sardy
M, Karpati S, Merkl B, Paulsson M, Smyth N, 2002: J. Exp. Med. 195, 6:
747-757. Epidermal Transglutaminase (Tgase 3) Is the Autoantigen of
Dermatitis Herpetiformis.
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Cotton
M, Stegmüller K, Eickhoff J, Hanke M, Herzberger K, Herget T, Choidas
A, Daub H, Godl K, 2003: NAR 31: 1-11. Exploiting features of
adenovirus replication to support mammalian kinase production.
Après
expression dans la levure
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Murphy
JT, Lagarias JC, 1997: Photochem. Photobiol. 65: 750-758. Purification
and characterization of recombinant affinity peptide-tagged oat
phytochrome A.
Après
expression dans des cellules de plante
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Drucker
M, German-Retana S, Espérandieu P, LeGall O, Blanc S, 2002: BTi, June:6-18.
Purification of a Viral Protein From Infected Plant Tissues Using The
Strep-tag.
Protéines
complexes
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Kleymann
G, Ostermeier C, Ludwig B, Skerra A, Michel H, 1995: Bio/Technology
13: 155-160. Engineered Fv fragments as a tool for the one-step
purification of integral multisubunit membrane protein complexes.
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Tsiotis
G, Haase W, Engel A, Michel H, 1995: Eur. J. Biochem. 231: 823-830.
Isolation and structural characterization of trimeric cyanobacterial
photosystem I complex with the help of recombinant antibody fragments.
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Zwicker
N, Adelhelm K, Thiericke R, Grabley S, Hanel F, 1999: Biotechniques
27: 368-375. Strep-tag II for one-step affinity purification of active
bHLHzip domain of human c-Myc.
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Cotton
M, Stegmüller K, Eickhoff J, Hanke M, Herzberger K, Herget T, Choidas
A, Daub H, Godl K, 2003: NAR 31: 1-11. Exploiting features of
adenovirus replication to support mammalian kinase production.
Enzymes
- métalloenzymes
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Hans
M, Buckel W, Bill E, 2000: Eur. J. Biochem. 267, 7082-7093.The
iron-sulfur clusters of 2-hydroxyglutaryl-CoA dehydratase from
Acidaminococcus fermentans. Spectroscopic and biochemical
investigations
-
Laber
B, Maurer W, Scharf S, Stepusin K, Schmidt FS, 1999: FEBS Lett. 449:
45-48. Vitamin B6 biosynthesis: formation of pyridoxine 5'-phosphate
from 4-(phosphohydroxy)-L-threonine and 1-deoxy-D-xylulose-5-phosphate
by PdxA and PdxJ protein.
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Hans
M, Buckel W, 2000: Biotech International, September issue: 12.
Purification of recombinant component A of 2-hydroxyglutaryl-CoA
dehydratase from Acidaminococcus fermentans using Strep-Tactin
affinity chromatography.
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Maier
T, Drapal N, Thanbichler M, Böck A, 1998: Anal. Biochem. 259: 68-73.
Strep-tag II affinity purification: an approach to study intermediates
of metalloenzyme biosynthesis.
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Wendt
UK, Wenderoth I, Tegeler A, von Schaewen A, 2000: The Plant Journal
23: 723-733. Molecular characterization of a novel glucose-6-phosphate
dehydrogenase from potato (Solanum tuberosum L.).
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Busch
K, Piehler J, Fromm H, 2000: Biochemistry 39: 10110-10117. Plant
succinic semialdehyde dehydrogenase: dissection of nucleotide binding
by surface plasmon resonance and fluorescence spectroscopy.
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Juda
GA, Bollinger JA, Dooley DM, 2001: Protein Expression and Purification
22: 455-461. Construction, over expression, and purification of
Arthrobacter globiformis amine oxidase-Strep-tag II fusion protein.
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Kohlstock
UF, Rücknagel KP, Reuter M, Schierhorn A, Andreesen JR, Söhling B,
2001: Eur. J. Biochem. 268: 6417-6425. Cys359 of GrdD is the
active-site thiol that catalyses the final step of acetyl phosphate
formation by glycin reductase from Eubacterium acidaminophilum.
-
Fontaine
L, Meynial-Salles I, Girbal L, Yang X, Croux C, Soucaille P, 2002: J.
Bacteriol. 184: 821-830. Molecular characterization and
transcriptional analysis of adhE2, the gene encoding the
NADH-dependent aldehyde/alcohol dehydrogenase responsible for butanol
production in alcohologenic cultures of Clostridium acetobutylicum
ATCC 824.
-
Roberts
SA, Weichsel A, Grass G, Thakali K, Hazzard JT, Tollin G, Rensing C,
Montfort WR, 2002: Proc. Natl. Acad. Sci. U S A 99: 2766-2771. Crystal
structure and electron transfer kinetics of CueO, a multicopper
oxidase required for copper homeostasis in Escherichia coli.
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Hengsakul
M, Cass AEG, 1997: J. Mol. Biol. 266: 621-632. Alkaline
phosphatase-Strep-tag fusion protein binding to streptavidin: Resonant
mirror studies.
Sécrétion
-
Tudyka
T, Skerra A, 1997: Protein Sci. 6: 2180-2187. Glutathione
S-transferase can be used as a C-terminal, enzymatically active
dimerization module for a recombinant protease inhibitor, and
functionally secreted into the periplasm of Escherichia coli.
Protéines
membranaires
-
Kleymann
G, Ostermeier C, Ludwig B, Skerra A, Michel H, 1995: Bio/Technology
13: 155-160. Engineered Fv fragments as a tool for the one-step
purification of integral multisubunit membrane protein complexes.
-
Goldberg
M, Pribyl T, Juhnke S, Nies DH, 1999: JBC 274: 26065-26070. Energetics
and resistance-nodulation-cell division protein family.
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Tsiotis
G, Haase W, Engel A, Michel H, 1995: Eur. J. Biochem. 231: 823-830.
Isolation and structural characterization of trimeric cyanobacterial
photosystem I complex with the help of recombinant antibody fragments.
-
Loferer
H, Hammar M, Normark S, 1997: Molecular Microbiology 26: 11-23.
Availability of the fibre subunit CsgA and the nucleator protein CsgB
during assembly of fibronectin-binding curli is limited by the
intracellular concentration of the novel lipoprotein CsgG.
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Ostermeier
C, Harrenga A, Ermler U, Michel H, 1997: Proc. Natl. Acad. Sci. USA
94: 10547-10553. Structure at 2.7 Å resolution of the Paracoccus
denitrificans two-subunit cytochrome c oxidase complexed with an
antibody Fv fragment.
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Rübenhagen
R, Rönsch H, Jung H, Krämer R, Morbach S, 2000: JBC 275: 735-741.
Osmosensor and osmoregulator properties of the betaine carrier BetP
from Corynebacterium glutamicum in proteoliposomes.
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Bungert
S, Krafft B, Schlesinger R & Friedrich T, 1999: FEBS Letters 460:
207-211. One-step purification of the NADH dehydrogenase fragment of
the Echerichia coli complex I by means of Strep-tag affinity
chromatography.
Crystallisation
-
Locher
KP, Hans M, Yeh AP, Schmid B, Buckel W, Rees DC, 2001: J. Mol.Biol.
307, 297-308. Crystal Structure of the Acidaminococcus fermentans
2-Hydroxyglutaryl-CoA Dehydratase Component A
-
Roberts
SA, Weichsel A, Grass G, Thakali K, Hazzard JT, Tollin G, Rensing C,
Montfort WR, 2002: Proc. Natl. Acad. Sci. U S A 99: 2766-2771. Crystal
structure and electron transfer kinetics of CueO, a multicopper
oxidase required for copper homeostasis in Escherichia coli.
-
Ilk
N, Vollenkle C, Egelseer EM, Breitwieser A, Sleytr UB, Sara M, 2002:
Appl. Environ. Microbiol. 68: 3251-3260. Molecular characterization of
the S-layer gene, sbpA, of Bacillus sphaericus CCM 2177 and production
of a functional S-layer fusion protein with the ability to
recrystallize in a defined orientation while presenting the fused
allergen.
-
Ostermeier
C, Essen LO, Michel H, 1995: Proteins 21: 74-77. Crystals of an
antibody Fv fragment against an integral membrane protein diffracting
to 1.28 Å resolution.
-
Ostermeier
C, Iwata S, Ludwig B, Michel H, 1995: Nature Struct. Biol. 2: 842-846.
Fv fragment-mediated crystallization of the membrane protein bacterial
cytochrome c oxidase.
-
Ostermeier
C, Harrenga A, Ermler U, Michel H, 1997: Proc. Natl. Acad. Sci. USA
94: 10547-10553. Structure at 2.7 Å resolution of the Paracoccus
denitrificans two-subunit cytochrome c oxidase complexed with an
antibody Fv fragment.
Anticalins
et fragments d’anticorps
-
Beste
G, Schmidt FS, Stibora T, Skerra A, 1999: Proc. Natl. Acad. Sci. U S A
96: 1898-1903. Small antibody-like proteins with prescribed ligand
specificities derived from the lipocalin fold.
-
Schlehuber
S, Skerra A, 2001: Biol. Chem. 382: 1335-1342. Duocalins: engineered
ligand-binding proteins with dual specificity derived from the
lipocalin fold.
-
Rau
D, Kramer K, Hock B, 2002: Anal. Bioanal. Chem. 372: 261-267. Cloning,
functional expression and kinetic characterization of
pesticide-selective Fab fragment variants derived by molecular
evolution of variable antibody genes.
-
Brown
JC, Brown BA 2nd, Li Y, Hardin CC, 1998: Biochemistry 37: 16338-16348.
Construction and characterization of a quadruplex DNA selective
single-chain autoantibody from a viable motheaten mouse hybridoma with
homology to telomeric DNA binding proteins.
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Koo
K, Foegeding PM, Swaisgood HE, 1998: Appl Environ Microbiol 64:
2490-2496. Construction and expression of a bifunctional single-chain
antibody agains Bacillus cereus spores.
Double
tag, Strep-tag/6xHis-tag
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Fiedler
M, Horn C, Bandtlow C, Schwab ME, Skerra A, 2002: Protein Eng. Nov;15(11):931-41.
An engineered IN-1 F(ab) fragment with improved affinity for the
Nogo-A axonal growth inhibitor permits immunochemical detection and
shows enhanced neutralizing activity.
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Boettner
M, Prinz B, Holz C, Stahl U, Lang C. High-throughput screening for
expression of heterologous proteins in the yeast Pichia pastoris. J
Biotechnol. 2002 Oct 9;99(1):51-62.
-
Holz
C, Hesse O, Bolotina N, Stahl U, Lang C. A micro-scale process for
high-throughput expression of cDNAs in the yeast Saccharomyces
cerevisiae. Protein Expr Purif. 2002 Aug;25(3):372-8.
Purification
de protéine en production élevée (High-throughput)
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Sebastian
P, Wallwitz J, Schmidt S. Semi automated production of a set of
different recombinant GST-Streptag fusion proteins. J Chromatogr B
Analyt Technol Biomed Life Sci. 2003 Mar 25;786(1-2):343-55.
"We have chosen the Strep-tag for its unique features: high
selectivity, mild elution and the possibility to directly regenerate
the chromatography resin."
D-
Détection
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Kleymann
G, Ostermeier C, Heitmann K, Haase W, Michel H, 1995: The Journal of
Histochemistry and Cytochemistry 43: 607-614. Use of antibody
fragments (Fv) in immunochemistry.
-
Kleymann
G, Iwata S, Wiesmüller HH, Ludwig B, Michel H, 1995: Eur. J. Biochem.
230: 359-363. Immunoelectronic microscopy and epitope mapping with
monoclonal antibodies suggests the existence of an additional
N-terminal transmembrane helix in the cytochrome b subunit of
bacterial ubiquinol:cytochrome-c
oxidoreductases.
-
Tsiotis
G, Haase W, Engel A, Michel H, 1995: Eur. J. Biochem. 231: 823-830.
Isolation and structural characterization of trimeric cyanobacterial
photosystem I complex with the help of recombinant antibody fragments.
-
Ribrioux
S, Kleymann G, Haase W, Heitmann K, Ostermeier C, Michel H, 1996: The
Journal of Histochemistry and Cytochemistry 44: 207-213. Use of
nanogold- and fluorescent-labeled antibody Fv fragments in
immunochemistry.
E- Dosage
-
57.
Skerra A, Schmidt TGM, 2000: Meth. Enzymol. 326: 271-304. Use of the
Strep-tag and streptavidin for recombinant protein purification and
detection.
-
58.
Panke O, Gumbiowski K, Junge W, Engelbrecht S, 2000: FEBS Letters 472:
34-38. F-ATPase: specific observation of the rotating c subunit
oligomer of EF(o)EF(1).
-
59.
Ernst W J, Spenger A, Toellner L, Katinger H & Grabherr R M, 2000:
Eur. J. Biochem. 267: 4033-4039. Expanding baculovirus surface
display: Modification of the native coat protein gp64 of Autographa
californica NPV
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