With Panomics New TransLucent Reporter Vectors, you can monitor and even quantify the binding activity of transcription factors. Each TransLucent Reporter Vector contains a cis-acting DNA binding element that is recognized by a specific TF. Binding at this site results in the expression of firefly luciferase, an enzyme capable of catalyzing a powerful bioluminescent reaction. Light emitted from the chemical reaction is directly proportional to the amount of expressed enzyme and thus the binding activity of the targeted TF.
Like our EMSA gel-shift kits, the Panomics Reporter Vectors are ideal for validating the results obtained using TranSignal Protein/DNA Arrays. But while EMSA only allows in vitro verification of those results, the use of the TransLucent Reporter Vectors allows TF activity to be monitored in living cells. Don't settle for answers from the test tube; investigate TF binding where it happens!
TransLucent Reporter Construct
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The TransLucent Reporter Vectors have been specially constructed to report the binding activity of a single TF. Multiple copies of the cis-acting enhancer element have been inserted into each vector upstream of a minimal TA promoter and the TATA box from the Herpes simplex virus thymidine kinase promoter. This promoter sequence drives expression of the luciferase gene (luc). The backbone of the vector contains an ampicillin resistance gene for cloning purposes, an origin of replication, and an f1 origin for single-stranded DNA production. |
The cis-acting enhancer element sequence resides between the NheI and BglII restriction sites, upstream of the TATA box promoter, which drives expression of the firefly luciferase reporter gene upon transcription factor binding. Unique restriction sites are listed on the map. The length of the enhancer element differs for each TransLucent Reporter Vector, therefore the positions indicated on the vector map should be adjusted accordingly. All of the TransLucent Vectors contain the Panomics signature sequence. These vectors are intended for research use only and should not be used commercially. |
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