Advantages of using smaller particle size
- Improved Efficiency
- Lower Collection Volume
- Higher Loading Capacity
Phthalates purification on 25 g Silica column
(125 mg Dimethyl Phthalate + 125 mg Diethyl Phthalate)
in 1 ml injection
Cyclohexane - Ethyl Acetate (85% - 15 %)
Flow rate : 30 ml/min
UV : 254nm
Flow rate impact on peak resolution
Gradient elution :
A) Hexane B) Ethyl acetate
0 - 100% B in 7 minutes
12 g column at 17, 30, 45, 60 ml/min
Methoxy acetophenone, Methyl paraben
Achieve greater Productivity @ Higher Flow rate with Smaller Particles
Phthalates purification on 25 g silica column
(125 mg Dimethyl Phthalate + 125 mg Diethyl Phthalate)
in 1 ml injection
Cyclohexane - Ethyl Acetate Gradient
100 % CH : 1 min
80 % CH : 7 min
80 % CH : 13 min
Selection Guide for Silica Columns
Analysis conditions :
Eluent : cyclohexane / ethyl acetate 90/10 - Flow rate : 40 ml/min
| Std type - Irregular Silica 40/63 µm 40 g column |
HP type - High Performance Silica 50 µm 40 g column |
HC type - High Capacity Silica 50 µm 40 g column |
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| Load 0 5 ml of solution A |
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| Load 1 2 ml of solution C |
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| Load 2 5 ml of solution C |
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| Load 3 10 ml of solution C |
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| Solution A | Weight | Volume | Solvent |
|---|---|---|---|
| tri tertio butyl benzene | 3 g | 100 ml | Cyclohexane / ethyl acetate 90 /10 |
| Diethyl phthalate | 3 g | 100 ml | |
| Dimethyl phthalate | 3 g | 100 ml |
| Solution C | Weight | Volume | Solvent |
|---|---|---|---|
| tri tertio butyl benzene | 2 g | 100 ml | Cyclohexane / ethyl acetate 90 /10 |
| Diethyl phthalate | 40 g | 100 ml | |
| Dimethyl phthalate | 40 g | 100 ml |
Higher purity, in 7 minutes or 24 minutes - How much do you have ?
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| 50 µm puriflash Silica Std, 25 g P/N : IR-50SI/25G FlowRate : 20 ml/min Pressure : 0 bar Solvents : A : Hexane - B : Ethyl acetate Gradient CV : 0.0 - 9.6 CV, O - 20% B Sample : 2 ml, 500 mg/ml, 4% Load |
50 µm puriflash Silica HP, 25 g P/N : IR-50SIHP/25G FlowRate : 20 ml/min Pressure : 0 bar Solvents : A : Hexane - B : Ethyl acetate Gradient CV : 0.0 - 4,8 CV, O - 20% B ; 4.8 - 5.6 CV, 20% B Gradient time : 0:00 - 12:00,0 - 20% B ; 12:00 - 15:00, 20% B Sample : 2 ml, 500 mg/ml, 4% Load |
30 µm puriflash Silica HP, 25 g P/N : PF-30SIHP/25g FlowRate : 30 ml/min Pressure : 1 bar Solvents : A : Hexane - B : Ethyl acetate Gradient CV : 0.0 - 4.8 CV, O - 20% B;4.8 - 5.6 CV, 20% B Gradient time : 0:00 - 8:00,0 - 20% B ; 8:00 - 10:00, 20% B Sample : 2 ml, 500 mg/ml, 4% Load |
15 µm puriflash Silica HP, 25 g P/N : PF-15SIHP/25G FlowRate : 40 ml/min Pressure : 2 bars Solvents : A : Hexane - B : Ethyl acetate Gradient CV : 0.0 - 4.8 CV, O - 20% B ;4.8 - 5.6 CV, 20% B Gradient time : 0:00 - 6:00,0 - 20% B ; 6:00 - 7:00, 20% B Sample : 2 ml, 500 mg/ml, 4% Load |
Higher Capacity - 20 % sample load, 5 g of sample on a 25 g column !
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| 50 µm puriflash Silica HC, 25 g P/N : IR-50SIHC/25G FlowRate : 20 ml/min Pressure : 0 bar Solvents : A : Hexane - B : Ethyl acetate Gradient CV : 0.0 - 4.8 CV, O - 20% B ; 4.8 - 6.4 CV, 20% B Gradient time : 0:00 - 12:00,0 - 20% B ; 12:00 - 16:00, 20% B Sample : 5 ml, 500 mg/ml, 2.5 g, 10% Load |
50 µm puriflash Silica HP, 25 g P/N : IR-50SIHC/25G FlowRate : 20 ml/min Pressure : 0 bar Solvents : A : Hexane - B : Ethyl acetate Gradient CV : 0.0 - 4,8 CV, O - 20% B ; 4.8 - 6.6 CV, 20% B Gradient time : 0:00 - 12:00,0 - 20% B ; 12:00 - 16:00, 20% B Sample : 7 ml, 500 mg/ml, 3.5 g, 14% Load |
50 µm puriflash Silica HC, 25 g P/N : PF-50SIHC/25g FlowRate : 20 ml/min Pressure : 0 bar Solvents : A : Hexane - B : Ethyl acetate Gradient CV : 0.0 - 4.8 CV, O - 20% B;4.8 - 6.4 CV, 20% B Gradient time : 0:00 - 12:00,0 - 20% B ; 12:00 - 16:00, 20% B Sample : 10 ml, 500 mg/ml, 5.0 g, 20% Load |
MONTLUCON - 211 bis avenue J.F.Kennedy
BP 1140 - 03103 Montluçon cedex
Tél. (33) (0) 4 70 03 88 55 - Fax (33) (0) 4 70 03 82 60
www.interchim.com - interchim@interchim.com