HPLC

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Uptisphere Applications

Carotenoïds isomers by SFC

Uptisphere 120 Å 5µ C18-ODB, 250 x 4.6 mm

Hydrocortisone butyrate

Uptisphere 120 Å 3µ C18-HDO, 150 x 3.0 mm

Biogenic amines (after dansyl derivatization)

Uptisphere 120 Å 5µ C18-HDO, 150 x 2.0 mm

Guajakol probe for Zeolite

Uptisphere 120 Å 3µ C18-HDO, 150 x 3.0 mm

Catecholamines

Uptisphere 120 Å 3µ C18-ODB, 125 x 3.9 mm

Catecholamins from CD1 Mouse Brain Structure

Uptisphere 120 Å 3µ C18-ODB, 150 x 4.6 mm

Catechol derivatives, Indol & Salicylate

Uptisphere 120 Å 3µ C18-ODB, 150 x 4.6 mm

Carotenoïds isomers by SFC

Uptisphere 120 Å 5µ C18-ODB, 250 x 4.6 mm

1 - Zeaxanthine

2 - 9 cis + unknow

3 - beta all Trans

4 - 13 cis

5 - 15 cis

UP5ODB-25QS

1 - Zeaxanthine

2 - 9 cis

3 - unknow

4 - beta all Trans + 13 cis

5 - 15 cis

UP5HDO-25QS

1 - Zeaxanthine

2 - 9 cis + unknow

3 - beta all Trans

4 - 13 cis

5 - 15 cis

UP5HSC-25QS

1 - Zeaxanthine

2 - 9 cis + unknow

3 - beta all Trans

4 - 13 cis

5 - 15 cis

UP5NEC-25QS

Hydrocortisone butyrate

Uptisphere 120 Å 3µ C18-HDO, 150 x 3.0 mm

1 - Hydrocortisone

2 - Hydrocortison 21-butyrat

3 - Hydrocortison 17-butyrat

UP3HDO$15QS

ACN H₂O (44/56)

UV : 232 nm

1 - Hydrocortison

2 - Hydrocortison 21-butyrat

3 - Hydrocortison 17-butyrat

UP3HDO$15QS

Courtesy of VDS Optilabs

Biogenic amines (after dansyl derivatization)

Uptisphere 120 Å 5µ C18-HDO, 150 x 2.0 mm

10.38 Tryptamine

12.46 Phenethylamine

14.61 Putrescine

15.89 Cadaverine

18.29 Histamine

19.54 1.7 DH

22.43 Tyramine

24.26 Spermidine

27.63 Spermine

UP5HDO#15QS

A : H2O-MeOH (1/3 - 2/3)

B : ACN-MeOH (50/50)

A to B in 30 min

PDA 336 nm

Courtesy of iFBM

Guajakol probe for Zeolite

Catecholamines

Uptisphere 120 Å 3µ C18-HDO, 150 x 3.0 mm

1 - 2,2’-bipyridil

2 - Guajakol

3 - 2.3-dihydroxynaphthalene

MeOH H2O (80/20)

Flow rate : 1 ml/min

UV : 254 nm

UP3HDO$15QS

Courtesy of VDS Optilabs

6.04 Noradrenaline

7.78 Adrenaline

9.36 Internal Std

UP3ODB*12QS

Flow rate : 0.60 ml/min

Injection : 10 µl

Catecholamins from CD1 Mouse Brain Structure

Catechol derivatives, Indol & Salicylate

Uptisphere 120 Å 3µ C18-ODB, 150 x 4.6 mm

Striatum + cortex + hippocampe

Uptisphere 120 Å 3µ C18-ODB, 150 x 4.6 mm

from Sprague-Dawley Rats Brains Dialysis

5.13 3.4 DBHA (internal Std)

6.56 DA

8.30 DOPAC

12.72 3-MT

14.68 5-HIAA

15.96 5-HT

17.88 HVA

UP3ODB-15QK

(Na2HPO4 50 mmoles/I + EDTA 2.69 mmoles/I + Pic B7 1.25mmoles/I)

MeOH 13 % v/v - pH 3.95 - Flow rate : 0.9 ml/min - Inj. 40 µl at 8°C-

Column temperature : 24 °C - Electrochemical detection (E1:100 mV- E2 : + 450 mV)

3.39 DOPA

4.80 3.4 DHBA (Internal Std)

6.44 DA

7.53 2.5 DHBA

8.60 DOPAC

10.42 2.3 DHBA

13.60 5-HIAA

14.48 5-HT

21.27 HVA

UP3ODB-15QK

(Na2HPO4 50 mmoles/l + EDTA 2.69 mmoles/l + Pic B7 1.25 mmoles/l)

MeOH 13% v/v - pH : 3.15 - Flow rate : 1 ml/min - Inj : 10 µl at 8 °C

Column temperature : 40 °C

Electrochemical detection (E1 : - 100mV ; E2 : + 180 mV)

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