HPLC

 

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Uptisphere Applications

 

 

Polyether of fatty Acids

 

Competitor

Uptisphere 300 Å 5 µ WOD, 250 x 4.6 mm

Hydrosoluble vitamins

Uptisphere 120 Å 5µ C18-HDO, 250 x 4.6 mm

Uptisphere 120 Å 5µ C18-ODB, 150 x 4.6 mm

Vitamins from serum extract

Uptisphere 120 Å 5µ C18-ODB, 250 x 4.6 mm

OPA Amino acids

Uptisphere 120 Å 5µ C18-HDO, 250 x 4.6 mm

Amino acids

 

Uptisphere 120 Å 5µ C18-HDO, 250 x 4.6 mm

Uptisphere 120 Å 3µ C18-HDO, 150 x 4.6 mm

Oil cuts separation on DNAP

Arjuna (terrestrial o.m.)

Boscan (marine o.m)

Thiols determination by mBBr technic

Uptisphere 120 Å 5µ C18-HDO, 250 x 4.6 mm

 

Polyether of fatty Acids

Competitor

MeOH 70%

(H2O + 0.1% TFA) 30%

Uptisphere 300 Å 5µ WOD, 250 x 4.6 mm

 

 

MeOH 75%

(H2O + 0.1% TFA) 25%

split mode

 

 

 

UP5WOD-25QS 

Hydrosoluble vitamins

Hydrosoluble vitamins

Uptisphere 120 Å 5µ C18-HDO, 250 x 4.6 mm

Uptisphere 120 Å 5µ C18-ODB, 150 x 4.6 mm

 

4.5 B1,NO3

5.8 C

7.6 PP

11.3 B6,HCl

13.8 B5

17 B2 base

 

UP5HDO-25QS

 

 

 

 

A : ACN

B : 0.05M Buffer (pH : 2,6)

0 to 20 min : 0% A

20 to 23 min : 40% A

23 to 40 min : 0% A

B : 0.05M Buffer (pH : 2,6)

0 to 20 min : 0% A

20 to 23 min : 40% A

23 to 40 min : 0% A

 

1- Riboflavin

2- Nicotinamide

3- Pyridoxim

 

UP5ODB-15QS

 

MeOH H3PO4 0.1% (50/50) : 49%

Heptane sulfonate 0.016 M

+ 1 ml H3PO4 : 51%

0.7 ml/min

 

 

Vitamins from serum extract

OPA Amino acids

Uptisphere 120 Å 5µ C18-ODB, 250 x 4.6 mm

Uptisphere 120 Å 5µ C18-HDO, 250 x 4.6 mm

UP5ODB-25QS

5.65 Vitamin A

7.61 Ac retinol

15.93 Vitamin E

MeOH 100%

1 ml/min

UV 295 nm

1-L-cysteic acid

2-ASP

3-Glu

4-ASN

5-His

6-SER

7-Gln

8-ARG

9-Gly

10-THR

 

11-Taurine

12-Ala

13-Tyr

14-Met

15-Trp

16-Val

17-Phe

18-Ile

19-Leu

20-Lys

UP5HDO-25K

A : (50 mM Acetate Na pH : 5.7) ; 3% THF

B : 100% MeOH ; 0.05 THF

Flow : 1 ml/min ; 37 °C - inj : 250 pmole of each AA

Fluo : ex : 346 nm . em : 455 nm

Amino acids

Amino acids

Uptisphere 120 Å 5µ C18-HDO, 250 x 4.6 mm

Uptisphere 120 Å 3µ C18-HDO, 150 x 4.6 mm

UP5HDO-25QS

Analysis time for the last peak : b- Lys : 56.55 min

Benefits :

• same resolution

• increased productivity

• less solvent consumption

UP3HDO-15QS

Analysis time for the last peak : b- Lys : 31.58 min

 

Oil cuts separation on DNAP

Arjuna (terrestrial o.m.)

Boscan (marine o.m)

7-10 min: monoaromatics

10-18 min: C3/C4 naphtalenes

18-25 min: C1 fluorenes dibenzothiophenes

25-28 min: phenanthrene

0-11 min : hexane 0.5 ml/min

11-15 min : hexane 1 ml/min

15-25 min : 1 ml/min gradient hexane /DCM 80/20

25-35 min : 1 ml/min gradient DCM 100

UV detection 254 nm (GC/MS identification)

 

UP40DNAP-25QS

 

Courtesy of Mme Vandenbroucke IFP

 

Thiols determination by mBBr technic

Uptisphere 120 Å 5µ C18-HDO, 250 x 4.6 mm

UP5HDO-25K

A : (Na Perchlorate 7.03g) + ACN 5% + (Acetic acid 0.25%PH : 3.4 (NaOH))

B : (Na Perchlorate 7.03g) + ACN 80% + (Acetic acid 0.25%PH : 3.4 (NaOH))

Flow : 1 ml/min ; 37 °C - ex : 388 nm ; em : 480 nm

 

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