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Bio-Works, a Sweden company, designs, develops and manufactures a broad product range in Uppsala.
For uses within areas of Life Sciences, Food & Beverage. |
WorkBeads™ Protein A
WorkBeads™ BabyBio A
WorkBeads™ Protein A & BabyBio A are designed for the purification of monoclonal and polyclonal antibodies. Made from agarose, well established and well-known in the Biotech industry.
- Higher dynamic binding capacity for monoclonal antibodies; excellent recovery & high purity
- Stronger coupling chemistry; high pH stability and low leakage
- Reliable, reproducible and efficient
WorkBeads Protein A affinity chromatography media is produced from agarose using a cross-linking method that results in a highly porous and physically stable agarose matrix. Agarose matrices have successfully been used for many years in biotechnology research and in the industrial purification of proteins.
The recombinant protein A is engineered to facilitate an oriented coupling to the matrix which allows high binding capacities for target proteins. The high capacity, chemical stability and low ligand leakage make WorkBeads Protein A ideal for purification of mono- and polyclonal antibodies.
WorkBeads™ Protein A is designed to allow oriented coupling to the bead via multipoint attachment. This allows high utilization of the immobilized protein A resulting in high IgG binding conditions.
Bead has a dynamic binding capacity of typically more than 40 mg IgG/ml media under standard binding conditions (PBS, pH 7.4 and 3 minutes residence time).
Media Characteristics
Medium WorkBeads Protein A |
WorkBeads Protein A |
Target substance |
Antibodies (IgG), bound via the Fc-region |
Matrix |
Rigid, highly cross-linked agarose |
Ligand |
Recombinant protein A expressed in E. coli using animal-free medium |
Coupling chemistry |
Bromohydrin |
Dynamic binding capacity (DBC) |
>40 mg human IgG/ml medium |
Recommended flow rate |
250 cm/h |
Max flow rate conditions (see Data Sheet) |
500 cm/h |
Chemical stability |
Compatible with all standard aqueous buffers used for protein purification, and 10 mM HCl (pH2),10 mM NaOH (pH 12), 0.1M sodium citrate-HCl (pH 3), 6 M guanidine-HCl, 20% ethanol. Should not be stored at low pH for prolonged time. |
Recommended working range pH Stability |
3-10 short term
2-12 cleaning |
Storage |
+2°C to +8°C in 20% ethanol |
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