For fluorescently labeling (tagging) a gene of interest, is it necessary to culture transfected cells to dilute cells containing donor DNA?
FAQ from OriGene
If in the donor construct the fluorescent protein does not have a mammalian expression promoter (for example the left homologous arm does not contain the promoter) , then you can sort the fluorescent cells out (you can tell in donor DNA only transfected cells); you might not need to get individual cell colonies. If the fluorescent protein in the donor template DNA does contain a mammalian expression promoter, you will need to pass the transfected cells several generations to dilute cells containing the donor construct.
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