Storage before use:
Store the columns in a cool place, away from light and dust.
Do not remove the blue fittings until the column is required for use.
First use:
puriFlash® columns fittings are Luer-type.
Attach the bottom of the column to a female Luer connector.
Attach the top of the column to a male Luer-lock connector.
Column conditioning:
This stage is essential to benefit of the full performance of the column.
It activates the silica & expels the air present in the column.
For optimum purification, this stage must be performed before the sample is loaded.
Rinse the column with 3 to 5 column volumes of solvent.
The solvent chosen is generally the solvent used for the beginning of purification.
Warning: Conditioning with a strong eluent will result in poor separation due to the high affinity of the solvent with the compounds to be purified.
Drying the column after the conditioning stage results in column performance loss.
Loading the sample:
There are two main sample loading techniques:
- Liquid injection:
The sample must be soluble with the eluent used for the beginning of purification or with a less eluting solvent.
It is dissolved in minimum solvent to prevent peak broadening (dispersion phenomenon).
- Solid deposit:
The sample is insoluble or sparingly soluble in the primary solvent.
1 to 2 g of silica per g of raw sample is introduced into a flask.
A solvent is added to dissolve the entire sample.
The mixture is evaporated under vacuum until a dry residue is obtained.
The mixture is loaded into a solid deposit module.
We recommand to carefully rid off air from the dryload by flushing it with an appropriate solvent before coupling to the column.
Operating pressure:
Never exceed the operating pressure limit values specified on the column.
Storage after use:
The columns may be either stored in an appropriate solvent for a period of approximately one week, with the exception of hexane & cyclohexane (for which the shelf-life should not exceed one day) or dried immediately prior to disposal.
Warning : Used columns must be reprocessed according to the applicable standards for this type of waste.