You will find here many useful products for IP and WB-IP applications, such as :
almost 2000 pairs of antibodies for IP-WB
Please also click here for a complete listing of all anti-IgG, Light Chain Specific antibody conjugates.
Anti IgG, Light Chain specific
When labeled secondary antibodies specific for both heavy and light chains of IgG, e.g. anti-IgG (H+L), are used to detect protein bands on Western blots following immunoprecipitation (IP), two dense bands appear (Figure A) corresponding to the heavy (50 kDa) and light chains (25 kDa) of the precipitated primary antibody. These bands usually obscure detection of any protein of interest with a molecular weight near 50 kDa or 25 kDa. However, when labeled anti-IgG, Light Chain Specific antibodies are used for detection, they bind only to the light chain band on the blot (Figure B) and to light chains on the native primary antibodies used for detection. Therefore, a 50 kDa protein may be detected on blots without interference from the precipitated IgG in the same band by incubation with its unlabeled primary antibody followed by labeled anti-IgG,Light Chain Specific. Similarly, a 25 kDa protein may be detected after IP without interference by using its unlabeled primary antibody followed by labeled anti-IgG, Fc fragment specific.