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LIFE SCIENCES > Immunodetection Tools > Accessory Reagents > Blotter Systems

The Miniblotter™ instruments enable you to perform multiple antibody or probe incubations on a single membrane, using microliter volumes of reagent. Typical applications include monoclonal antibody screening on western blots, animal and human serum screening, and DNA probe hybridizations.  Sample processing is streamlined through use of the Washing Manifold, which enables washing of all sample lanes simultaneously. Reactions can be detected by all conventional methods, including enzyme conjugates, chemiluminescence and radiolabels.

 

  • For membrane assays including Western, Northern, and Southern Blots and Checker-board Blots
  • Requires microliter volumes, not milliliter volumes
  • Multiple samples analyzed simultaneously
  • No cross-contamination between channels
  • Decrease processing time
  • All results on a single membrane, non need to strip
  • Compatible with all standard electrophoresis systems

Product Cat# Cat# (with washing manifold) Chanels/samples per blot Max rgt vol. Dimensions
channels
Blots
dimensions
Manifold Divers
Miniblotter 45 Unit (for regular gel processing)
987900 538370 45 channels 110µL 1.5 mm x 13 cm 13 cm F2 (a)
Miniblotter 25 Unit (for regular gel processing)
BN4550 661060 25 channels 250µL 4.0 mm x 13 cm 13 cm F2 -
Miniblotter 28 - Single Unit (for mini gel processing)
S31580 590550 28 channels 50µL 1.5 mm x 5.5 cm 8.3 cm F1 (b)
Miniblotter 28 - Dual Unit (for mini gel processing)
168830 649400 2 x 28 channels 50µL 1.5 mm x 5.5 cm 8.3 cm F1 (b)
Miniblotter 20 - Single Unit (for mini gel processing)
BN4580 572090 20 channels 350µL 3.0 mm x 5.5 cm 8.3 cm F1 -
Miniblotter 16 - Dual Unit
S31580 590550 2 x 16 channels 350µL 3.0 mm x 5.5 cm 8.3 cm F1 (c)
 
(a) Combs available for gel electrophoresis to line up Miniblotter channels with electrophoresis lane
(b) Ideal for hybridoma screening from 96-well microplates. Hybridoma supernatants can be transferred directly into Miniblotter 28 channels using an 8-channel automatic pipette.
(c) Similar to Miniblotter 20 - Single Unit, except that there are two sets of 16 channels so that two membrane blots can be placed between the Top and Bottom Plates allowing for the processing of up to 32 samples (16x2).

Disposable Miniblot Systems for Minigel Processing

The Disposable Miniblot System operates exactly as the Miniblotter 28 unit, but its Top Plate frame (penned ´C-Shell´) holds a disposable cassette. In addition to a test-specific blot, this disposable plastic cassette offers the same sample ports and 28 (enclosed) reaction channels as the Miniblotter 28 Unit. In the Disposable Miniblot System, however, once the assay is completed and the blot has been developed, the disposable cassette is thrown away - minimizing operator contact with potentially harmful reagents.

 
  • Cassettes prevent risk of carry-over from one assay to another.
  • Cassettes eliminate possibility of cross-contamination between samples
  • Cassettes safely contain potentially hazardous serum during assay

Cassette Miniblot - Single Unit Cat# (dual unit) Chanels/samples per blot Max rgt vol. Dimensions
channels
Blots
dimensions
Manifold Divers
Cassette Miniblot - Single Unit
982720 - 28 channels 50µL 1.5 mm x 6 cm 8.3 cm F1 (b)
Cassette Miniblot - Dual Unit
982721 - 2 x 28 channels 50µL 1.5 mm x 6 cm 8.3 cm F1 (b)
 
Please inquire for other blotting accessories (rapid downward transfer system Turboblotter #BN478, standard incubation trays #BN480, sealable hybridization folders #BN481,..).

 

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